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Literature summary extracted from

  • Cui, L.; Du, G.; Zhang, D.; Liu, H.; Chen, J.
    Purification and characterization of transglutaminase from a newly isolated Streptomyces hygroscopicus (2007), Food Chem., 105, 612-618.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.2.13 ethanol enzyme activity is slightly elevated at a lower concentration of ethanol at 25°C. With increasing ethanol concentration, the enzyme activity decreases, and in the presence of 50% ethanol the enzyme activity is almost lost after incubation for 30 min at 25°C Streptomyces hygroscopicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.13 5,5'-dithiobis (2-nitrobenzoic acid)
-
 Streptomyces hygroscopicus
2.3.2.13 Cu2+ 5 mM CuSO4, 91% inhibition Streptomyces hygroscopicus
2.3.2.13 Hg2+ 5 mM HgCl2, 94% inhibition Streptomyces hygroscopicus
2.3.2.13 additional information the enzyme is not inhibited by ethylenediaminetetraacetic acid, Na+, K+, Ca2+, Mg2+, Ba2+, Mn2+ and Co2+ Streptomyces hygroscopicus
2.3.2.13 Pb2+ 5 mM Pb(CH3COO)2, 80% inhibition Streptomyces hygroscopicus
2.3.2.13 Zn2+ 5 mM ZnSO4, 95% inhibition Streptomyces hygroscopicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.13 0.05469
-
 N-carboxybenzoyl-L-glutaminyl-glycine pH 6.0, 37°C Streptomyces hygroscopicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.13 38000
-
 x * 38000, SDS-PAGE Streptomyces hygroscopicus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.3.2.13 Ethanol stability is strongly affected by ethanol concentration Streptomyces hygroscopicus

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.13 Streptomyces hygroscopicus
-
 WSH03-13
-
2.3.2.13 Streptomyces hygroscopicus WSH03-13
-
 WSH03-13
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.13
-
 Streptomyces hygroscopicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.13 culture medium
-
 Streptomyces hygroscopicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.2.13 7.6
-
-
 Streptomyces hygroscopicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.13 N-carboxybenzoyl-L-glutaminyl-glycine + hydroxylamine
-
 Streptomyces hygroscopicus hydroxamate + ?
-
 ?
2.3.2.13 N-carboxybenzoyl-L-glutaminyl-glycine + hydroxylamine
-
 Streptomyces hygroscopicus WSH03-13 hydroxamate + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.13 ? x * 38000, SDS-PAGE Streptomyces hygroscopicus

Synonyms

EC Number Synonyms Comment Organism
2.3.2.13 TGase
-
 Streptomyces hygroscopicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.2.13 37 45 at pH 6.0, hydroxamate formation from N-carboxybenzoyl-L-glutaminyl-glycine and hydroxylamine Streptomyces hygroscopicus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.2.13 30 50 at pH 6.0, 30°C: about 70% of maximal activity, 50°C: about 70% of maximal activity Streptomyces hygroscopicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.2.13 30
-
 30 min, pH 6.0, about 10% loss of activity Streptomyces hygroscopicus
2.3.2.13 40
-
 30 min, pH 6.0, about 20% loss of activity Streptomyces hygroscopicus
2.3.2.13 50
-
 pH 6.0, 30 min, about 45% loss of activity Streptomyces hygroscopicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.13 6 7 hydroxamate formation from N-carboxybenzoyl-L-glutaminyl-glycine and hydroxylamine Streptomyces hygroscopicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.2.13 5 8 pH 5.0: about 80% of maximal activity, pH 8.0: about 80% of maximal activity Streptomyces hygroscopicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.3.2.13 3.5
-
 30 min, 50% loss of activity Streptomyces hygroscopicus
2.3.2.13 5 8 30 min, stable at 10°C Streptomyces hygroscopicus
2.3.2.13 9
-
 30 min, 20% loss of activity Streptomyces hygroscopicus