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Literature summary extracted from

  • Velez, H.; Glassbrook, N.J.; Daub, M.E.
    Mannitol biosynthesis is required for plant pathogenicity by Alternaria alternata (2008), FEMS Microbiol. Lett., 285, 122-129.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.138 additional information MtDH transcript increases after treatment with tobacco leaf extract Alternaria alternata
1.1.1.138 additional information transcript of both MtDH and MPDH start to increase by 30 min after the addition of tobacco extract, reaching 2.5fold to almost 4fold higher by 2 h. No increase in transcript for MtDH and MPDH if cultures are treated with water instead of tobacco leaf extract Alternaria alternata

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.17 additional information construction of a disruption mutant, that shows elevated levels of trehalose, but is viable, and the conidia germinate normally, the mutant is less pathogenic in infected tobacco plants compared to the wild-type fungus, overview Alternaria alternata
1.1.1.138 additional information MtDH mutant produces less mannitol than compared to the wild-type. The MtDH/MPDH double mutant does not produce mannitol. Response of the genes in the disruption mutants is similar to that in wild-type. Conidia of mutants are viable and germinated normally. Elevated levels of trehalose in the mutants, suggesting that trehalose may substitute for mannitol as a storage compound for germination. Tobacco inoculation shows no reduction in lesion severity caused by the MtDH mutant as compared with wild-type, whereas the MtDH/MPDH double mutant causes significantly less disease. The double mutant is unaffected in the ability to germinate and produce appressoria on tobacco leaves and elicites a defense response from the host, indicating that it is able to penetrate and infect the host Alternaria alternata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.17 D-mannitol 1-phosphate + NAD+ Alternaria alternata the enzyme is involved in mannitol biosynthesis, which is required for plant pathogenicity by Alternaria alternata, overview D-fructose 6-phosphate + NADH + H+
-
 r
1.1.1.17 D-mannitol 1-phosphate + NAD+ Alternaria alternata A5 the enzyme is involved in mannitol biosynthesis, which is required for plant pathogenicity by Alternaria alternata, overview D-fructose 6-phosphate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.17 Alternaria alternata
-
-
-
1.1.1.17 Alternaria alternata A5
-
-
-
1.1.1.138 Alternaria alternata
-
-
-
1.1.1.138 Alternaria alternata A5
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.17 D-mannitol 1-phosphate + NAD+
-
 Alternaria alternata D-fructose 6-phosphate + NADH + H+
-
 r
1.1.1.17 D-mannitol 1-phosphate + NAD+ the enzyme is involved in mannitol biosynthesis, which is required for plant pathogenicity by Alternaria alternata, overview Alternaria alternata D-fructose 6-phosphate + NADH + H+
-
 r
1.1.1.17 D-mannitol 1-phosphate + NAD+
-
 Alternaria alternata A5 D-fructose 6-phosphate + NADH + H+
-
 r
1.1.1.17 D-mannitol 1-phosphate + NAD+ the enzyme is involved in mannitol biosynthesis, which is required for plant pathogenicity by Alternaria alternata, overview Alternaria alternata A5 D-fructose 6-phosphate + NADH + H+
-
 r
1.1.1.138 D-mannitol + NAD(P)+
-
 Alternaria alternata D-fructose + NAD(P)H + H+
-
 ?
1.1.1.138 D-mannitol + NAD(P)+
-
 Alternaria alternata A5 D-fructose + NAD(P)H + H+
-
 ?
1.1.1.138 D-mannitol + NADP+
-
 Alternaria alternata D-fructose + NADPH + H+
-
 r
1.1.1.138 D-mannitol + NADP+
-
 Alternaria alternata A5 D-fructose + NADPH + H+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.17 mannitol 1-phosphate 5-dehydrogenase
-
 Alternaria alternata
1.1.1.17 MPDH
-
 Alternaria alternata
1.1.1.138 MtDH
-
 Alternaria alternata

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.17 NAD+
-
 Alternaria alternata
1.1.1.17 NADH
-
 Alternaria alternata
1.1.1.138 NADP+
-
 Alternaria alternata