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Literature summary extracted from

  • Mustakhimov, I.I.; Rozova, O.N.; Reshetnikov, A.S.; Khmelenina, V.N.; Murrell, J.C.; Trotsenko, Y.A.
    Characterization of the recombinant diaminobutyric acid acetyltransferase from Methylophaga thalassica and Methylophaga alcalica (2008), FEMS Microbiol. Lett., 283, 91-96.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.178 into the pET22b+ vector for expression in Escherichia coli BL21DE3 cells Methylophaga thalassica
2.3.1.178 into the pET22b+ vector for expression in Escherichia coli BL21DE3 cells Methylophaga alcalica

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.178 Ca2+
-
 Methylophaga alcalica
2.3.1.178 Ca2+
-
 Methylophaga thalassica
2.3.1.178 Cd2+
-
 Methylophaga alcalica
2.3.1.178 Cd2+
-
 Methylophaga thalassica
2.3.1.178 Cu2+
-
 Methylophaga alcalica
2.3.1.178 Cu2+
-
 Methylophaga thalassica
2.3.1.178 Mg2+
-
 Methylophaga alcalica
2.3.1.178 Mg2+
-
 Methylophaga thalassica
2.3.1.178 Ni2+
-
 Methylophaga alcalica
2.3.1.178 Ni2+
-
 Methylophaga thalassica
2.3.1.178 Zn2+
-
 Methylophaga alcalica
2.3.1.178 Zn2+
-
 Methylophaga thalassica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.178 0.37
-
 L-2,4-diaminobutanoate
-
 Methylophaga thalassica
2.3.1.178 0.375
-
 L-2,4-diaminobutanoate
-
 Methylophaga alcalica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.178 19700
-
 calculated molecular mass Methylophaga thalassica
2.3.1.178 20000
-
 calculated molecular mass Methylophaga alcalica
2.3.1.178 20000
-
 determined by gel filtration and SDS-PAGE Methylophaga thalassica
2.3.1.178 20000
-
 determined by gel filtration and SDS-PAGE Methylophaga alcalica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.178 acetyl-CoA + L-2,4-diaminobutanoate Methylophaga thalassica
-
 CoA + N4-acetyl-L-2,4-diaminobutanoate
-
 ?
2.3.1.178 acetyl-CoA + L-2,4-diaminobutanoate Methylophaga alcalica
-
 CoA + N4-acetyl-L-2,4-diaminobutanoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.178 Methylophaga alcalica A9YZY0
-
-
2.3.1.178 Methylophaga thalassica A9YZX5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.178 using a Ni2+-nitrilotriacetic acid-agarose and a Blue Sepharose 6FF column Methylophaga thalassica
2.3.1.178 using a Ni2+-nitrilotriacetic acid-agarose and a Blue Sepharose 6FF column Methylophaga alcalica

Storage Stability

EC Number Storage Stability Organism
2.3.1.178 4°C, 50 mM Tris-HCl, pH 8.5, 1 month Methylophaga thalassica
2.3.1.178 4°C, 50 mM Tris-HCl, pH 8.5, 1 month Methylophaga alcalica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.178 acetyl-CoA + L-2,4-diaminobutanoate
-
 Methylophaga thalassica CoA + N4-acetyl-L-2,4-diaminobutanoate
-
 ?
2.3.1.178 acetyl-CoA + L-2,4-diaminobutanoate
-
 Methylophaga alcalica CoA + N4-acetyl-L-2,4-diaminobutanoate
-
 ?
2.3.1.178 acetyl-CoA + L-2,4-diaminobutanoate
-
 Methylophaga thalassica CoA + (2S)-4-acetamido-2-aminobutanoate
-
 ?
2.3.1.178 acetyl-CoA + L-2,4-diaminobutanoate
-
 Methylophaga alcalica CoA + (2S)-4-acetamido-2-aminobutanoate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.178 homodimer
-
 Methylophaga thalassica
2.3.1.178 homodimer
-
 Methylophaga alcalica

Synonyms

EC Number Synonyms Comment Organism
2.3.1.178 DABAAT
-
 Methylophaga alcalica
2.3.1.178 diaminobutyrate acetyltransferase
-
 Methylophaga thalassica
2.3.1.178 diaminobutyrate acetyltransferase
-
 Methylophaga alcalica
2.3.1.178 diaminobutyric acid acetyltransferase
-
 Methylophaga thalassica
2.3.1.178 diaminobutyric acid acetyltransferase
-
 Methylophaga alcalica
2.3.1.178 EctA
-
 Methylophaga thalassica
2.3.1.178 EctA
-
 Methylophaga alcalica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.178 30
-
 activity assay Methylophaga thalassica
2.3.1.178 30
-
 activity assay Methylophaga alcalica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.178 8.5 9
-
 Methylophaga thalassica
2.3.1.178 9.5
-
-
 Methylophaga alcalica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.178 7 9.5
-
 Methylophaga thalassica
2.3.1.178 7 10.5
-
 Methylophaga alcalica