BRENDA - Enzyme Database show

NB-protein (BchN-BchB) of dark-operative protochlorophyllide reductase is the catalytic component containing oxygen-tolerant Fe-S clusters

Nomata, J.; Ogawa, T.; Kitashima, M.; Inoue, K.; Fujita, Y.; FEBS Lett. 582, 1346-1350 (2008)

Data extracted from this reference:

Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.3.1.33
Fe2+
contains two 4Fe-4S clusters
Rhodobacter capsulatus
1.3.1.33
Mg2+
required for activity
Rhodobacter capsulatus
1.3.7.7
Fe2+
DPOR contains 7.23 mol Fe2+ per NB-protein
Rhodobacter capsulatus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.3.1.33
Rhodobacter capsulatus
-
-
-
1.3.7.7
Rhodobacter capsulatus
-
-
-
Oxidation Stability
EC Number
Oxidation Stability
Organism
1.3.7.7
the NB-protein activity persists after the exposure to air for almost 2 h
Rhodobacter capsulatus
Purification (Commentary)
EC Number
Commentary
Organism
1.3.1.33
NB-protein component from Pchlide oxidoreductase
Rhodobacter capsulatus
1.3.7.7
protochlorophyllide-saturated NB-protein from DPOR is purified by Strep-Tactin Sepharose column chromatography
Rhodobacter capsulatus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.3.7.7
0.0262
-
purified enzyme, pH not specified in the publication, at 34°C
Rhodobacter capsulatus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.1.33
protochlorophyllide + NADPH + H+
-
686758
Rhodobacter capsulatus
chlorophyllide + NADP+
-
-
-
?
1.3.7.7
protochlorophyllide + oxidized ferredoxin + ADP + phosphate
-
686758
Rhodobacter capsulatus
chlorophyllide a + reduced ferredoxin + ATP
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.3.1.33
NADPH
-
Rhodobacter capsulatus
1.3.7.7
4Fe-4S-center
NB-protein of DPOR carries two oxygen-tolerant [4Fe–4S] clusters
Rhodobacter capsulatus
1.3.7.7
ADP
-
Rhodobacter capsulatus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.3.1.33
NADPH
-
Rhodobacter capsulatus
1.3.7.7
4Fe-4S-center
NB-protein of DPOR carries two oxygen-tolerant [4Fe–4S] clusters
Rhodobacter capsulatus
1.3.7.7
ADP
-
Rhodobacter capsulatus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.3.1.33
Fe2+
contains two 4Fe-4S clusters
Rhodobacter capsulatus
1.3.1.33
Mg2+
required for activity
Rhodobacter capsulatus
1.3.7.7
Fe2+
DPOR contains 7.23 mol Fe2+ per NB-protein
Rhodobacter capsulatus
Oxidation Stability (protein specific)
EC Number
Oxidation Stability
Organism
1.3.7.7
the NB-protein activity persists after the exposure to air for almost 2 h
Rhodobacter capsulatus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.3.1.33
NB-protein component from Pchlide oxidoreductase
Rhodobacter capsulatus
1.3.7.7
protochlorophyllide-saturated NB-protein from DPOR is purified by Strep-Tactin Sepharose column chromatography
Rhodobacter capsulatus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.3.7.7
0.0262
-
purified enzyme, pH not specified in the publication, at 34°C
Rhodobacter capsulatus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.1.33
protochlorophyllide + NADPH + H+
-
686758
Rhodobacter capsulatus
chlorophyllide + NADP+
-
-
-
?
1.3.7.7
protochlorophyllide + oxidized ferredoxin + ADP + phosphate
-
686758
Rhodobacter capsulatus
chlorophyllide a + reduced ferredoxin + ATP
-
-
-
?