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Literature summary extracted from

  • Nomata, J.; Ogawa, T.; Kitashima, M.; Inoue, K.; Fujita, Y.
    NB-protein (BchN-BchB) of dark-operative protochlorophyllide reductase is the catalytic component containing oxygen-tolerant Fe-S clusters (2008), FEBS Lett., 582, 1346-1350.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.1.33 Fe2+ contains two 4Fe-4S clusters Rhodobacter capsulatus
1.3.1.33 Mg2+ required for activity Rhodobacter capsulatus
1.3.7.7 Fe2+ DPOR contains 7.23 mol Fe2+ per NB-protein Rhodobacter capsulatus

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.33 Rhodobacter capsulatus
-
-
-
1.3.7.7 Rhodobacter capsulatus
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.3.7.7 the NB-protein activity persists after the exposure to air for almost 2 h Rhodobacter capsulatus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.33 NB-protein component from Pchlide oxidoreductase Rhodobacter capsulatus
1.3.7.7 protochlorophyllide-saturated NB-protein from DPOR is purified by Strep-Tactin Sepharose column chromatography Rhodobacter capsulatus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.7.7 0.0262
-
 purified enzyme, pH not specified in the publication, at 34°C Rhodobacter capsulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.33 protochlorophyllide + NADPH + H+
-
 Rhodobacter capsulatus chlorophyllide + NADP+
-
 ?
1.3.7.7 protochlorophyllide + oxidized ferredoxin + ADP + phosphate
-
 Rhodobacter capsulatus chlorophyllide a + reduced ferredoxin + ATP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.33 dark-operative protochlorophyllide oxidoreductase consists of the two components, L-protein (BchL-dimer) and NB-protein (BchN-BchBheterotetramer) Rhodobacter capsulatus
1.3.1.33 Pchlide oxidoreductase
-
 Rhodobacter capsulatus
1.3.7.7 dark-operative Pchlide oxidoreductase
-
 Rhodobacter capsulatus
1.3.7.7 dark-operative protochlorophyllide reductase
-
 Rhodobacter capsulatus
1.3.7.7 DPOR
-
 Rhodobacter capsulatus
1.3.7.7 light-independent protochlorophyllide oxidoreductase
-
 Rhodobacter capsulatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.33 NADPH
-
 Rhodobacter capsulatus
1.3.7.7 4Fe-4S-center NB-protein of DPOR carries two oxygen-tolerant [4Fe–4S] clusters Rhodobacter capsulatus
1.3.7.7 ADP
-
 Rhodobacter capsulatus