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Literature summary extracted from

  • Szabo, J.; Varga, A.; Flachner, B.; Konarev, P.V.; Svergun, D.I.; Zavodszky, P.; Vas, M.
    Role of side-chains in the operation of the main molecular hinge of 3-phosphoglycerate kinase (2008), FEBS Lett., 582, 1335-1340.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.7.2.3 E192A kcat/Km for ATP is 11.7fold lower than wild-type enzyme, kcat/3-phosphoglycerate is 58.6fold lower than wild-type value Homo sapiens
2.7.2.3 F165A kcat/Km for ATP is 23fold lower than wild-type enzyme, kcat/3-phosphoglycerate is 60.7fold lower than wild-type value Homo sapiens
2.7.2.3 F196A kcat/Km for ATP is 4.2fold lower than wild-type enzyme, kcat/3-phosphoglycerate is 19.3fold lower than wild-type value Homo sapiens
2.7.2.3 S392A kcat/Km for ATP is 1.03fold lower than wild-type enzyme, kcat/3-phosphoglycerate is 9.4fold lower than wild-type value Homo sapiens
2.7.2.3 S392A/T393A kcat/Km for ATP is 304fold lower than wild-type enzyme, kcat/3-phosphoglycerate is 1717fold lower than wild-type value Homo sapiens
2.7.2.3 S398A kcat/Km for ATP is 1.13fold lower than wild-type enzyme, kcat/3-phosphoglycerate is 5.3fold lower than wild-type value Homo sapiens
2.7.2.3 T393A kcat/Km for ATP is 113fold lower than wild-type enzyme, kcat/3-phosphoglycerate is 288fold lower than wild-type value Homo sapiens
2.7.2.3 T393del kcat/Km for ATP is 14615fold lower than wild-type enzyme, kcat/3-phosphoglycerate is 27869fold lower than wild-type value Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.3 0.0005
-
 3-phospho-D-glyceroyl phosphate wild-type enzyme Homo sapiens
2.7.2.3 0.0091
-
 3-phospho-D-glyceroyl phosphate mutant enzyme E192A Homo sapiens
2.7.2.3 0.01
-
 3-phospho-D-glyceroyl phosphate mutant enzyme T393A Homo sapiens
2.7.2.3 0.017
-
 3-phospho-D-glyceroyl phosphate mutant enzyme F165A Homo sapiens
2.7.2.3 0.03
-
 ATP mutant enzyme S398A Homo sapiens
2.7.2.3 0.05
-
 3-phospho-D-glycerate wild-type enzyme Homo sapiens
2.7.2.3 0.062
-
 3-phospho-D-glycerate mutant enzyme S398A Homo sapiens
2.7.2.3 0.078
-
 ATP mutant enzyme S392A Homo sapiens
2.7.2.3 0.09
-
 ADP mutant enzyme E192A Homo sapiens
2.7.2.3 0.1
-
 ADP mutant enzyme T393A Homo sapiens
2.7.2.3 0.11
-
 ATP wild-type enzyme Homo sapiens
2.7.2.3 0.11
-
 3-phospho-D-glycerate mutant enzyme T393del Homo sapiens
2.7.2.3 0.12
-
 ADP wild-type enzyme Homo sapiens
2.7.2.3 0.12
-
 ADP mutant enzyme F165A Homo sapiens
2.7.2.3 0.13
-
 ATP mutant enzyme T393del Homo sapiens
2.7.2.3 0.24
-
 ATP mutant enzyme E192A Homo sapiens
2.7.2.3 0.32
-
 3-phospho-D-glycerate mutant enzyme S392A Homo sapiens
2.7.2.3 0.33
-
 ATP mutant enzyme F196A Homo sapiens
2.7.2.3 0.53
-
 3-phospho-D-glycerate mutant enzyme E192A Homo sapiens
2.7.2.3 0.66
-
 ATP mutant enzyme S392A/T393A Homo sapiens
2.7.2.3 0.69
-
 3-phospho-D-glycerate mutant enzyme F196A Homo sapiens
2.7.2.3 0.83
-
 ATP mutant enzyme F165A Homo sapiens
2.7.2.3 0.86
-
 ATP mutant enzyme T393A Homo sapiens
2.7.2.3 0.95
-
 3-phospho-D-glycerate mutant enzyme F165A Homo sapiens
2.7.2.3 0.99
-
 3-phospho-D-glycerate mutant enzyme T393A Homo sapiens
2.7.2.3 1.66
-
 3-phospho-D-glycerate mutant enzyme S392A/T393A Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.3 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.3 ADP + 3-phospho-D-glyceroyl phosphate
-
 Homo sapiens ATP + 3-phospho-D-glycerate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.2.3 3-phosphoglycerate kinase
-
 Homo sapiens
2.7.2.3 hPGK
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.2.3 0.067
-
 ATP mutant enzyme T393del Homo sapiens
2.7.2.3 0.067
-
 3-phospho-D-glycerate mutant enzyme T393del Homo sapiens
2.7.2.3 16.4
-
 ATP mutant enzyme S392A/T393A Homo sapiens
2.7.2.3 16.4
-
 3-phospho-D-glycerate mutant enzyme S392A/T393A Homo sapiens
2.7.2.3 58
-
 ATP mutant enzyme T393A Homo sapiens
2.7.2.3 58
-
 3-phospho-D-glycerate mutant enzyme T393A Homo sapiens
2.7.2.3 155
-
 ATP mutant enzyme E192A Homo sapiens
2.7.2.3 155
-
 3-phospho-D-glycerate mutant enzyme E192A Homo sapiens
2.7.2.3 200
-
 ATP mutant enzyme S398A Homo sapiens
2.7.2.3 200
-
 3-phospho-D-glycerate mutant enzyme S398A Homo sapiens
2.7.2.3 250
-
 ADP mutant enzyme T393A Homo sapiens
2.7.2.3 250
-
 3-phospho-D-glyceroyl phosphate mutant enzyme T393A Homo sapiens
2.7.2.3 270
-
 ATP mutant enzyme F165A Homo sapiens
2.7.2.3 270
-
 3-phospho-D-glycerate mutant enzyme F165A Homo sapiens
2.7.2.3 575
-
 ATP mutant enzyme S392A Homo sapiens
2.7.2.3 575
-
 3-phospho-D-glycerate mutant enzyme S392A Homo sapiens
2.7.2.3 610
-
 ATP mutant enzyme F196A Homo sapiens
2.7.2.3 610
-
 3-phospho-D-glycerate mutant enzyme F196A Homo sapiens
2.7.2.3 833
-
 ATP wild-type enzyme Homo sapiens
2.7.2.3 833
-
 3-phospho-D-glycerate wild-type enzyme Homo sapiens
2.7.2.3 1130
-
 ADP mutant enzyme E192A Homo sapiens
2.7.2.3 1130
-
 3-phospho-D-glyceroyl phosphate mutant enzyme E192A Homo sapiens
2.7.2.3 1170
-
 ADP mutant enzyme F165A Homo sapiens
2.7.2.3 1170
-
 3-phospho-D-glyceroyl phosphate mutant enzyme F165A Homo sapiens
2.7.2.3 2630
-
 ADP wild-type enzyme Homo sapiens
2.7.2.3 2630
-
 3-phospho-D-glyceroyl phosphate wild-type enzyme Homo sapiens