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Literature summary extracted from

  • Noge, K.; Kato, M.; Mori, N.; Kataoka, M.; Tanaka, C.; Yamasue, Y.; Nishida, R.; Kuwahara, Y.
    Geraniol dehydrogenase, the key enzyme in biosynthesis of the alarm pheromone, from the astigmatid mite Carpoglyphus lactis (Acari: Carpoglyphidae) (2008), FEBS J., 275, 2807-2817.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.183 DNA and amino acid sequence determination and analysis, phylogenetic analysis Carpoglyphus lactis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.183 0.051
-
 geraniol pH 9.0, 25°C Carpoglyphus lactis
1.1.1.183 0.0596
-
 NAD+ pH 9.0, 25°C Carpoglyphus lactis
1.1.1.347 0.051
-
 geraniol in 50 mM Tris-HCl (pH 8.5), at 22°C Carpoglyphus lactis
1.1.1.347 0.0595
-
 NAD+ in 50 mM Tris-HCl (pH 8.5), at 22°C Carpoglyphus lactis
1.1.1.354 0.051
-
 geraniol pH 8.5, 22°C Carpoglyphus lactis
1.1.1.354 0.0595
-
 NAD+ pH 8.5, 22°C Carpoglyphus lactis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.183 42800
-
 x * 42800, SDS-PAGE Carpoglyphus lactis
1.1.1.347 40630
-
 1 * 40630, calculated from amino acid sequence Carpoglyphus lactis
1.1.1.347 42800
-
 1 * 42800, active form, SDS-PAGE Carpoglyphus lactis
1.1.1.347 56200
-
 gel filtration Carpoglyphus lactis
1.1.1.354 40630
-
 1 * 40630, calculated from sequence Carpoglyphus lactis
1.1.1.354 42800
-
 1 * 42800, SDS-PAGE Carpoglyphus lactis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.183 geraniol + NAD+ Carpoglyphus lactis the enzyme plays an important role in the biosynthesis of neral, an alarm pheromone geranial + NADH + H+
-
 ?
1.1.1.347 geraniol + NAD+ Carpoglyphus lactis the enzyme selectively oxidizes geraniol geranial + NADH + H+
-
 ir
1.1.1.354 (2E,6E)-farnesol + NAD+ Carpoglyphus lactis key enzyme in biosynthesis of the alarm pheromone neral (2E,6E)-farnesal + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.183 Carpoglyphus lactis B2NI94 astigmatid mite
-
1.1.1.347 Carpoglyphus lactis
-
-
-
1.1.1.354 Carpoglyphus lactis B2NI94
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.183 native enzyme 1879fold to homogeneity by ammonium sulfate fractionation, followed by hydrophobic interactin and adsorption chromatography, anion exchange chromatography, and gel filtration in seven purification steps, overview Carpoglyphus lactis
1.1.1.347 ammonium sulfate precipitation, Toyopearl phenyl-650M column chromatography, Toyopearl HW-50F column chromatography, DEAE-cellulose column chromatography, Blue-Cellulofine column chromatography, MonoQ column chromatography and Superdex 200gel filtration Carpoglyphus lactis
1.1.1.354
-
 Carpoglyphus lactis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.183 31
-
 purified enzyme Carpoglyphus lactis
1.1.1.347 0.016
-
 crude enzyme, in 50 mM Tris-HCl (pH 8.5), at 22°C Carpoglyphus lactis
1.1.1.347 31
-
 after 1879fold purification, in 50 mM Tris-HCl (pH 8.5), at 22°C Carpoglyphus lactis
1.1.1.354 31
-
 pH 8.5, 22°C Carpoglyphus lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.183 E,E-farnesol + NAD+
-
 Carpoglyphus lactis ?
-
 ?
1.1.1.183 geraniol + NAD+ the enzyme plays an important role in the biosynthesis of neral, an alarm pheromone Carpoglyphus lactis geranial + NADH + H+
-
 ?
1.1.1.183 geraniol + NAD+ stereospecific reaction, no activity with nerol Carpoglyphus lactis geranial + NADH + H+
-
 ?
1.1.1.183 additional information substrate specificity, poor activity with citronellol, 3-methyl-2-buten-1-ol, 1-octanol, 1-butanol, 2-buten-1-ol, and ethanol, overview Carpoglyphus lactis ?
-
 ?
1.1.1.347 (2E,6E)-farnesol + NAD+ 50% activity compared to geraniol Carpoglyphus lactis (2E,6E)-farnesyl aldehyde + NADH + H+
-
 ?
1.1.1.347 3-methyl-2-buten-1-ol + NAD+ 4.3% activity compared to geraniol Carpoglyphus lactis 3-methyl-but-2-enal + NADH + H+
-
 ?
1.1.1.347 citronellol + NAD+ 4.3% activity compared to geraniol Carpoglyphus lactis citronellal + NADH + H+
-
 ?
1.1.1.347 geraniol + NAD+ the enzyme selectively oxidizes geraniol Carpoglyphus lactis geranial + NADH + H+
-
 ir
1.1.1.347 additional information NADP+ is ineffective as a cofactor. The enzyme does not use nerol as substrate. Straight-chain alcohols such as 1-octanol, 2-buten-1-ol, 1-butanol and ethanol serve as poor substrates Carpoglyphus lactis ?
-
 ?
1.1.1.354 (2E,6E)-farnesol + NAD+ key enzyme in biosynthesis of the alarm pheromone neral Carpoglyphus lactis (2E,6E)-farnesal + NADH + H+
-
 ?
1.1.1.354 (2E,6E)-farnesol + NAD+ 50% of the activity compared to geraniol Carpoglyphus lactis (2E,6E)-farnesal + NADH + H+
-
 ?
1.1.1.354 3-methyl-2-buten-1-ol + NAD+ 4.3% of the activity compared to geraniol Carpoglyphus lactis 3-methyl-2-butenal + NADH + H+
-
 ?
1.1.1.354 citronellol + NAD+ 4.3% of the activity compared to geraniol Carpoglyphus lactis ?
-
 ?
1.1.1.354 geraniol + NAD+ nerol, the geometrical isomer of geraniol is inactive. The enzyme cannot use NADP+ as cofactor Carpoglyphus lactis geranial + NADH + H+
-
 ?
1.1.1.354 additional information straight-chain alcohols such as 1-octanol, 2-buten-1-ol, 1-butanol and ethanol serve as poor substrates Carpoglyphus lactis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.183 ? x * 42800, SDS-PAGE Carpoglyphus lactis
1.1.1.347 monomer 1 * 40630, calculated from amino acid sequence Carpoglyphus lactis
1.1.1.347 monomer 1 * 42800, active form, SDS-PAGE Carpoglyphus lactis
1.1.1.354 monomer 1 * 40630, calculated from sequence Carpoglyphus lactis
1.1.1.354 monomer 1 * 42800, SDS-PAGE Carpoglyphus lactis

Synonyms

EC Number Synonyms Comment Organism
1.1.1.183 GeDH
-
 Carpoglyphus lactis
1.1.1.183 More the enzyme is a member of the medium-chain dehydrogenase/reductase family Carpoglyphus lactis
1.1.1.347 GeDH
-
 Carpoglyphus lactis
1.1.1.354 GeDH
-
 Carpoglyphus lactis
1.1.1.354 NAD+-farnesol dehydrogenase
-
 Carpoglyphus lactis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.183 25
-
-
 Carpoglyphus lactis
1.1.1.347 25
-
-
 Carpoglyphus lactis
1.1.1.354 25
-
-
 Carpoglyphus lactis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.183
-
 60 temprature profile, overview Carpoglyphus lactis
1.1.1.354 5 60 5°C: about 50% of maximal activity, 60°C: about 50% of maximal activity Carpoglyphus lactis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.347 2996
-
 geraniol in 50 mM Tris-HCl (pH 8.5), at 22°C Carpoglyphus lactis
1.1.1.354 2996
-
 geraniol pH 8.5, 22°C Carpoglyphus lactis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.183 9
-
-
 Carpoglyphus lactis
1.1.1.347 9
-
-
 Carpoglyphus lactis
1.1.1.354 9
-
-
 Carpoglyphus lactis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.347 7 10 as the pH increases from 7.0 to 7.5, there is a sharp increase in enzyme activity whereas the activity decreases sharply beyond pH 10.0 Carpoglyphus lactis
1.1.1.354 8 10.5 pH 8.0: about 75% of maximal activity, pH 10.5: about 35% of maximal activity Carpoglyphus lactis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.183 NAD+ specific for, no activity with NADP+ Carpoglyphus lactis
1.1.1.347 NAD+ NADP+ is ineffective as cofactor Carpoglyphus lactis
1.1.1.354 NAD+ the enzyme cannot use NADP+ as cofactor Carpoglyphus lactis

General Information

EC Number General Information Comment Organism
1.1.1.347 physiological function the enzyme plays an important role in the biosynthesis of neral, an alarm pheromone in Carpoglyphus lactis Carpoglyphus lactis
1.1.1.354 physiological function key enzyme in biosynthesis of the alarm pheromone neral Carpoglyphus lactis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.347 58800
-
 geraniol in 50 mM Tris-HCl (pH 8.5), at 22°C Carpoglyphus lactis
1.1.1.354 58400
-
 geraniol pH 8.5, 22°C Carpoglyphus lactis