BRENDA - Enzyme Database show

Amidase-catalyzed production of nicotinic acid in batch and continuous stirred membrane reactors

Cantarella, M.; Cantarella, L.; Gallifuoco, A.; Intellini, R.; Kaplan, O.; Spera, A.; Martinkova, L.; Enzyme Microb. Technol. 42, 222-229 (2008)
No PubMed abstract available

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
3.5.1.4
synthesis
amidase-catalyzed production of nicotinic acid in batch and continuous stirred membrane reactors
Microbacterium imperiale
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.4
Microbacterium imperiale
-
-
-
3.5.1.4
Microbacterium imperiale CBS 498-74
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.1.4
resting cell
-
Microbacterium imperiale
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.4
nicotinamide + H2O
-
686460
Microbacterium imperiale
nicotinic acid + NH3
-
-
-
?
3.5.1.4
nicotinamide + H2O
-
686460
Microbacterium imperiale CBS 498-74
nicotinic acid + NH3
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.1.4
60
-
-
Microbacterium imperiale
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.5.1.4
additional information
-
amidase-catalysed reaction provides an activation energy value of 52.6/53.5 kJ/mol
Microbacterium imperiale
3.5.1.4
45
65
45°C: about 45% of maximal activity, 65°C: about 70% of maximal activity
Microbacterium imperiale
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.1.4
50
-
half-life: 200 h
Microbacterium imperiale
3.5.1.4
60
-
half-life: 5 h
Microbacterium imperiale
Application (protein specific)
EC Number
Application
Commentary
Organism
3.5.1.4
synthesis
amidase-catalyzed production of nicotinic acid in batch and continuous stirred membrane reactors
Microbacterium imperiale
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.1.4
resting cell
-
Microbacterium imperiale
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.4
nicotinamide + H2O
-
686460
Microbacterium imperiale
nicotinic acid + NH3
-
-
-
?
3.5.1.4
nicotinamide + H2O
-
686460
Microbacterium imperiale CBS 498-74
nicotinic acid + NH3
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.1.4
60
-
-
Microbacterium imperiale
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.5.1.4
additional information
-
amidase-catalysed reaction provides an activation energy value of 52.6/53.5 kJ/mol
Microbacterium imperiale
3.5.1.4
45
65
45°C: about 45% of maximal activity, 65°C: about 70% of maximal activity
Microbacterium imperiale
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.5.1.4
50
-
half-life: 200 h
Microbacterium imperiale
3.5.1.4
60
-
half-life: 5 h
Microbacterium imperiale