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Literature summary extracted from

  • Lopez, M.S.; Perez, J.P.; Lopez-Cabarcos, E.; Lopez-Ruiz, B.
    Amperometric biosensors based on choline oxidase entrapped in polyacrylamide microgels (2007), Electroanalysis, 19, 370-378.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.1.3.17 biotechnology development and applications of biosensors Alcaligenes sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.17 Nicotine ability of nicotine to inhibit catalytic activity of choline oxidase used for biosensorial detection of Alcaligenes sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.17 additional information
-
 additional information similar level of catalytic activity than free enzyme in solution Alcaligenes sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.17 choline + O2 Alcaligenes sp.
-
 betaine aldehyde + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.17 Alcaligenes sp. P16101
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.17 commercial preparation
-
 Alcaligenes sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.17 additional information
-
 biosensor using choline oxidase entrapped within polyacrylamide microparticles, microgel analysis by Scanning electron microscopy, Galai-Cis particle analyzer, X-ray diffraction and differential scanning calorimetry, influence of the polymer cross-linking content, potential, pH and temperature the biosensor response investigated, design of a bienzyme biosensor by including acetylcholinesterase Alcaligenes sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.17 choline + O2
-
 Alcaligenes sp. betaine aldehyde + H2O2
-
 ?
1.1.3.17 choline + O2 choline detection by mono- and bienzyme biosensors Alcaligenes sp. betaine aldehyde + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.3.17 ChOx protein
-
 Alcaligenes sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.17 25
-
 oxygen saturation, 8.0 mM of choline, 0.1 M Tris-phosphate buffer, pH 9 Alcaligenes sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.3.17 18 30 similar to that of the free enzyme ranging between 25°C and 35°C Alcaligenes sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.17 9
-
 optimum for choline detection, biosensor response examined in 0.1 M phosphate/acetate/TRIS buffer from pH 4.0 to 10.0 at 25°C Alcaligenes sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.17 7.5 10 biosensor retains 60% of its maximum response Alcaligenes sp.