Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Gonzalez-Covarrubias, V.; Ghosh, D.; Lakhman, S.S.; Pendyala, L.; Blanco, J.G.
    A functional genetic polymorphism on human carbonyl reductase 1 (CBR1 V88I) impacts on catalytic activity and NADPH binding affinity (2007), Drug Metab. Dispos., 35, 973-980.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.184 expression in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.184 V88I functional genetic polymorphism. Mutation results in CBR1 isoforms with different binding affinities for cofactor NADPH and inhibitor rutin as well as different maximal velocities for reaction with daunorubicin and prostaglandin E2 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.184 0.042
-
 menadione wild-type, 37°C, pH 7.4 Homo sapiens
1.1.1.184 0.055
-
 menadione mutant V88I, 37°C, pH 7.4 Homo sapiens
1.1.1.184 0.14
-
 daunorubicin mutant V88I, 37°C, pH 7.4 Homo sapiens
1.1.1.184 0.173
-
 daunorubicin wild-type, 37°C, pH 7.4 Homo sapiens
1.1.1.184 0.247
-
 NADPH wild-type, 37°C, pH 7.4 Homo sapiens
1.1.1.184 0.309
-
 prostaglandin E2 wild-type, 37°C, pH 7.4 Homo sapiens
1.1.1.184 0.32
-
 prostaglandin E2 mutant V88I, 37°C, pH 7.4 Homo sapiens
1.1.1.184 0.343
-
 NADPH mutant V88I, 37°C, pH 7.4 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.184 32000
-
 x * 32000, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.184 Homo sapiens
-
 isoform CBR1
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.184 liver
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.184 daunorubicin + NADPH
-
 Homo sapiens daunorubicinol + NADP+
-
 ?
1.1.1.184 menadione + NADPH
-
 Homo sapiens ? + NADP+
-
 ?
1.1.1.184 prostaglandin E2 + NADPH
-
 Homo sapiens prostaglandin F2alpha + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.184 ? x * 32000, SDS-PAGE Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.184 18
-
 prostaglandin E2 mutant V88I, 37°C, pH 7.4 Homo sapiens
1.1.1.184 25
-
 prostaglandin E2 wild-type, 37°C, pH 7.4 Homo sapiens
1.1.1.184 61
-
 daunorubicin mutant V88I, 37°C, pH 7.4 Homo sapiens
1.1.1.184 85
-
 menadione mutant V88I, 37°C, pH 7.4 Homo sapiens
1.1.1.184 91
-
 daunorubicin wild-type, 37°C, pH 7.4 Homo sapiens
1.1.1.184 110
-
 menadione wild-type, 37°C, pH 7.4 Homo sapiens
1.1.1.184 149
-
 NADPH mutant V88I, 37°C, pH 7.4 Homo sapiens
1.1.1.184 179
-
 NADPH wild-type, 37°C, pH 7.4 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.184 NADP+
-
 Homo sapiens
1.1.1.184 NADPH
-
 Homo sapiens