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Literature summary extracted from

  • Singh, S.A.; Christendat, D.
    The DHQ-dehydroshikimate-SDH-shikimate-NADP(H) complex: insights into metabolite transfer in the shikimate pathway (2007), Cryst. Growth Des., 7, 2153-2160.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.25 expression in Escherichia coli Arabidopsis thaliana

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.25 in complex with shikimate, and in ternary complex with shikimate and NADP+ Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.25 H335A site-directed mutagenesis, tenfold decrease in kcat value Arabidopsis thaliana
1.1.1.25 N406A site-directed mutagenesis, very strong decrease in kcat value Arabidopsis thaliana
1.1.1.25 Q578L site-directed mutagenesis, sixtyfold decrease in kcat value Arabidopsis thaliana
1.1.1.25 Q582L site-directed mutagenesis, very strong decrease in kcat value Arabidopsis thaliana
1.1.1.25 T381A site-directed mutagenesis, more than twentyfold decrease in kcat value Arabidopsis thaliana
1.1.1.25 T381S site-directed mutagenesis, fourfold decrease in kcat value, slight decrease in Km value Arabidopsis thaliana
1.1.1.25 T407A site-directed mutagenesis, 6.5fold decrease in kcat value, increase in Km-value Arabidopsis thaliana
1.1.1.25 T422S site-directed mutagenesis, tenfold decrease in kcat value Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.25 0.062
-
 NADP+ mutant T381S, 22°C Arabidopsis thaliana
1.1.1.25 0.068
-
 NADP+ mutant H335A, 22°C Arabidopsis thaliana
1.1.1.25 0.0928
-
 NADP+ mutant T381A, 22°C Arabidopsis thaliana
1.1.1.25 0.107
-
 NADP+ mutant T422S, 22°C Arabidopsis thaliana
1.1.1.25 0.131
-
 NADP+ wild-type, 22°C Arabidopsis thaliana
1.1.1.25 0.156
-
 NADP+ mutant T407A, 22°C Arabidopsis thaliana
1.1.1.25 0.159
-
 NADP+ mutant Q78L, 22°C Arabidopsis thaliana
1.1.1.25 0.459
-
 shikimate mutant T381S, 22°C Arabidopsis thaliana
1.1.1.25 0.556
-
 shikimate mutant T381A, 22°C Arabidopsis thaliana
1.1.1.25 0.626
-
 shikimate mutant H335A, 22°C Arabidopsis thaliana
1.1.1.25 0.685
-
 shikimate wild-type, 22°C Arabidopsis thaliana
1.1.1.25 0.92
-
 shikimate mutant Q78L, 22°C Arabidopsis thaliana
1.1.1.25 1.022
-
 shikimate mutant T407A, 22°C Arabidopsis thaliana
1.1.1.25 1.09
-
 shikimate mutant T422S, 22°C Arabidopsis thaliana
1.1.1.25 4.466
-
 shikimate mutant Q582L, 22°C Arabidopsis thaliana
1.1.1.25 7.12
-
 shikimate mutant N406A, 22°C Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.25 Arabidopsis thaliana
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.25 shikimate + NADP+ = 3-dehydroshikimate + NADPH + H+ the pro-R hydrogen of the nicotinamide C4 is 3.35 A from the C3 of shikimate. The catalytic K385 and D423 residues are proximal to the C3-hydroxyl of shikimate, which is deprotonated in the oxidation reaction. The enzyme adopts a concave architecture that places the active sites in a face-to-face arrangement Arabidopsis thaliana
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.25 shikimate + NADP+
-
 Arabidopsis thaliana 3-dehydroshikimate + NADPH + H+
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.25 0.174
-
 shikimate mutant N406A, 22°C Arabidopsis thaliana
1.1.1.25 0.346
-
 shikimate mutant Q582L, 22°C Arabidopsis thaliana
1.1.1.25 4.51
-
 NADP+ mutant Q78L, 22°C Arabidopsis thaliana
1.1.1.25 7.3
-
 shikimate mutant Q78L, 22°C Arabidopsis thaliana
1.1.1.25 15.1
-
 NADP+ mutant T381A, 22°C Arabidopsis thaliana
1.1.1.25 16.8
-
 shikimate mutant T381A, 22°C Arabidopsis thaliana
1.1.1.25 34
-
 NADP+ mutant H335A, 22°C Arabidopsis thaliana
1.1.1.25 42
-
 shikimate mutant H335A, 22°C Arabidopsis thaliana
1.1.1.25 43.3
-
 NADP+ mutant T422S, 22°C Arabidopsis thaliana
1.1.1.25 46.3
-
 shikimate mutant T422S, 22°C Arabidopsis thaliana
1.1.1.25 52.1
-
 NADP+ mutant T407A, 22°C Arabidopsis thaliana
1.1.1.25 66.6
-
 shikimate mutant T407A, 22°C Arabidopsis thaliana
1.1.1.25 105
-
 NADP+ mutant T381S, 22°C Arabidopsis thaliana
1.1.1.25 114
-
 shikimate mutant T381S, 22°C Arabidopsis thaliana
1.1.1.25 399
-
 NADP+ wild-type, 22°C Arabidopsis thaliana
1.1.1.25 428
-
 shikimate wild-type, 22°C Arabidopsis thaliana