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Literature summary extracted from

  • Verma, N.; Kumar, K.; Kaur, G.; Anand, S.
    L-asparaginase: a promising chemotherapeutic agent (2007), Crit. Rev. Biotechnol., 27, 45-62.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.1 Lipid activates Tetrahymena pyriformis

Application

EC Number Application Comment Organism
3.5.1.1 analysis direct measurement of L-asparagine in human plasma samples through the use of Escherichia coli Lasparaginase in the soluble form is a major clinical application of this system Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.1
-
 Lupinus angustifolius
3.5.1.1
-
 Lupinus arboreus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.1 Fe2+
-
 Rhodotorula mucilaginosa
3.5.1.1 Pb2+
-
 Rhodotorula mucilaginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.1 0.7
-
 L-asparagine
-
 Mycolicibacterium phlei
3.5.1.1 2.8
-
 L-asparagine
-
 Erwinia sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.1 extracellular
-
 Rhodotorula toruloides
-
-
3.5.1.1 extracellular
-
 Cyberlindnera jadinii
-
-
3.5.1.1 intracellular
-
 Enterobacter cloacae 5622
-
3.5.1.1 microsome
-
 Tetrahymena pyriformis
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.1 Mg2+ activates Rhodotorula mucilaginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.1 33000
-
 x * 33000, SDS-PAGE Thermus thermophilus
3.5.1.1 120000
-
-
 Sphagnum fallax

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.1 additional information Escherichia coli L-asparaginase from Escherichia coli is more immuno-depressive and immunotoxic than that from Erwinia carotovora ?
-
 ?
3.5.1.1 additional information Pectobacterium carotovorum L-asparaginase from Escherichia coli is more immuno-depressive and immunotoxic than that from Erwinia carotovora ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Aliivibrio fischeri
-
-
-
3.5.1.1 Aspergillus niger
-
-
-
3.5.1.1 Aspergillus tamarii
-
-
-
3.5.1.1 Aspergillus terreus
-
-
-
3.5.1.1 Cyberlindnera jadinii
-
-
-
3.5.1.1 Enterobacter cloacae
-
-
-
3.5.1.1 Erwinia aroidea
-
 NRR LB-138
-
3.5.1.1 Erwinia aroidea NRR LB-138
-
 NRR LB-138
-
3.5.1.1 Erwinia sp.
-
-
-
3.5.1.1 Escherichia coli
-
 strain W and strain K-12
-
3.5.1.1 Lupinus angustifolius
-
-
-
3.5.1.1 Lupinus arboreus
-
-
-
3.5.1.1 Mycolicibacterium phlei
-
-
-
3.5.1.1 Nocardia asteroides
-
-
-
3.5.1.1 Pectobacterium carotovorum
-
-
-
3.5.1.1 Photobacterium leiognathi
-
-
-
3.5.1.1 Photobacterium phosphoreum
-
-
-
3.5.1.1 Pseudomonas aeruginosa
-
-
-
3.5.1.1 Pseudomonas aeruginosa 50071
-
-
-
3.5.1.1 Pseudomonas fluorescens
-
-
-
3.5.1.1 Pseudomonas putida
-
-
-
3.5.1.1 Rhodotorula mucilaginosa
-
-
-
3.5.1.1 Rhodotorula toruloides
-
-
-
3.5.1.1 Saccharomyces cerevisiae
-
-
-
3.5.1.1 Saccharomyces cerevisiae P38986
-
-
3.5.1.1 Serratia marcescens
-
-
-
3.5.1.1 Sphagnum fallax
-
-
-
3.5.1.1 Staphylococcus sp.
-
-
-
3.5.1.1 Tetrahymena pyriformis
-
-
-
3.5.1.1 Thermus thermophilus
-
-
-
3.5.1.1 Vibrio harveyi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.1
-
 Cyberlindnera jadinii
3.5.1.1
-
 Mycolicibacterium phlei
3.5.1.1
-
 Pseudomonas aeruginosa
3.5.1.1
-
 Sphagnum fallax
3.5.1.1
-
 Thermus thermophilus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.1 culture condition L-asparaginase-I is constitutive Saccharomyces cerevisiae
-
3.5.1.1 culture condition L-asparaginase-II is secreted in response to N starvation Saccharomyces cerevisiae
-
3.5.1.1 culture condition carbon sources such as sucrose, maltose, galactose, lactose, mannitol and mannose inhibit enzyme production. Exogenous cAMP in presence of carbon sources stimulates L-asparaginase enzyme production Staphylococcus sp.
-
3.5.1.1 culture condition sabourand dextrose broth yields maximum growth and maximum L-asparaginase production Nocardia asteroides
-
3.5.1.1 culture condition high L-asparaginase activity is found in cells cultured on L-fructose, D-galactose, sucrose or maltose, and in cells cultured on L-asparagine as the sole nitrogen source Enterobacter cloacae
-
3.5.1.1 flower bud
-
 Lupinus arboreus
-
3.5.1.1 leaf
-
 Lupinus arboreus
-
3.5.1.1 additional information the synthesis of L-asparaginase in Escherichia coli W and Escherichia coli K-12 is almost completely suppressed if glucose is added at a concentration of 0.5% to the growth medium. Organic acids and amino acids such as L-leucine and L-methionine enhance production of L-asparaginase. n-Dodecane at 6% increases cell concentration by 12.7% and production of L-asparaginase by 21% to give 60.8 IU/ml in the fermentation medium Escherichia coli
-
3.5.1.1 additional information a pH of 7.9, casein hydrolysate (3.11%) and corn-steep liquor (3.68%) are the most significant factors improving the enzyme production process Pseudomonas aeruginosa
-
3.5.1.1 additional information highest L-asparaginase production in 2% proline medium, the lowest L-asparaginase production levels is found in the presence of glutamine and urea as nitrogen sources Aspergillus tamarii
-
3.5.1.1 additional information the lowest L-asparaginase production levels is found in the presence of glutamine and urea as nitrogen sources Aspergillus terreus
-
3.5.1.1 additional information production of high levels of L-asparaginase in solid state fermentation, using agro-wastes from three leguminous crops (bran of Cajanus cajan, Phaseolus mungo, and Glycine max) Aspergillus niger
-
3.5.1.1 additional information enzyme activity is highest during the exponential phase of growth Rhodotorula mucilaginosa
-
3.5.1.1 root tip
-
 Lupinus arboreus
-
3.5.1.1 seed developing Lupinus arboreus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 L-asparagine + H2O
-
 Erwinia sp. L-aspartate + NH3
-
 ?
3.5.1.1 L-asparagine + H2O
-
 Mycolicibacterium phlei L-aspartate + NH3
-
 ?
3.5.1.1 additional information L-asparaginase from Escherichia coli is more immuno-depressive and immunotoxic than that from Erwinia carotovora Escherichia coli ?
-
 ?
3.5.1.1 additional information L-asparaginase from Escherichia coli is more immuno-depressive and immunotoxic than that from Erwinia carotovora Pectobacterium carotovorum ?
-
 ?
3.5.1.1 additional information does not hydrolyse L-glutamine Erwinia sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.1 ? x * 33000, SDS-PAGE Thermus thermophilus
3.5.1.1 hexamer
-
 Erwinia sp.
3.5.1.1 homodimer
-
 Rhodotorula toruloides

Synonyms

EC Number Synonyms Comment Organism
3.5.1.1 L-asparaginase-I
-
 Saccharomyces cerevisiae
3.5.1.1 L-asparaginase-II
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.1 30 32
-
 Staphylococcus sp.
3.5.1.1 37
-
-
 Rhodotorula toruloides

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.1 77
-
 the enzyme follows linear kinetics, even at 77°C Erwinia sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.1 50
-
 stable for 10 min Cyberlindnera jadinii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.1 6
-
-
 Cyberlindnera jadinii
3.5.1.1 6.4
-
-
 Rhodotorula toruloides
3.5.1.1 8.2
-
-
 Sphagnum fallax
3.5.1.1 8.6 8.8
-
 Staphylococcus sp.
3.5.1.1 8.6
-
-
 Thermus thermophilus
3.5.1.1 9.2
-
-
 Erwinia sp.