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Literature summary extracted from

  • Lushchak, V.I.; Husak, V.V.; Storey, K.B.
    Regulation of AMP-deaminase activity from white muscle of common carp Cyprinus carpio (2008), Comp. Biochem. Physiol. B, 149B, 362-369.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.4.6 ADP 6.1fold activation at 2 mM Cyprinus carpio
3.5.4.6 ATP 3.7fold activation at 2 mM Cyprinus carpio

General Stability

EC Number General Stability Organism
3.5.4.6 heating in the presence of low KCl (150 mM) results in a rapid loss in activity with only 25% of initial activity being left after 30 min, about half of the initial activity remains after heating for 30 min in 25 mM histidine solution, solution, incubation in imidazole or phosphate solutions results in greater stability over 30 min of heating with AMPD activity decreasing by about 20% Cyprinus carpio

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.6 Ca2+ at 10 mM Ca2+ inhibition of AMPD is 28% Cyprinus carpio
3.5.4.6 fluoride 8 mM fluoride reduce AMPD activity by 54% Cyprinus carpio
3.5.4.6 H2O2 treatment with 0.1 mM H2O2 reduces activity by half after about 50 min, 50% of activity is lost in about 25 min when AMPD is incubated with both iron and H2O2 Cyprinus carpio
3.5.4.6 K+ inhibitory at concentrations above 300 mM, at 500 mM 62% of initial activities is left Cyprinus carpio
3.5.4.6 Mg2+ at 10 mM Mg2+ inhibition of AMPD is 13% Cyprinus carpio
3.5.4.6 additional information incubation with 0.005 mM FeSO4 does not affect AMPD activity Cyprinus carpio
3.5.4.6 Na+ inhibitory at concentrations above 250 mM, at 500 mM 46% of initial activities is left Cyprinus carpio
3.5.4.6 phosphate 10 mM phosphate lower activity by 39% Cyprinus carpio

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.4.6 K+ activates at low concentrations of 100-150 mM, in the presence of 100 mM K+ enzyme activity is increased by 1.7fold Cyprinus carpio
3.5.4.6 Na+ activates at low concentrations of 100-150 mM, in the presence of 100 mM Na+ enzyme activity is increased by 2.4fold Cyprinus carpio

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.6 55000
-
 subunit, SDS-PAGE Cyprinus carpio

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.6 Cyprinus carpio
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.6 phosphocellulose column chromatography Cyprinus carpio

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.4.6 gill
-
 Cyprinus carpio
-
3.5.4.6 kidney
-
 Cyprinus carpio
-
3.5.4.6 skeletal muscle higher activity in white muscle compared to red muscle Cyprinus carpio
-

Storage Stability

EC Number Storage Stability Organism
3.5.4.6 2-4°C, 1 M KCl, at least 1 month, no loss of activity Cyprinus carpio

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.6 AMP + H2O
-
 Cyprinus carpio IMP + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.4.6 AMP-aminohydrolase
-
 Cyprinus carpio
3.5.4.6 AMP-deaminase
-
 Cyprinus carpio
3.5.4.6 AmpD
-
 Cyprinus carpio

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.4.6 50
-
 heating at 50°C quickly destroys AMPD activity when the enzyme is incubated in distilled water, AMPD remains fully active over 30 min in the presence 1 M KCl at 50°C Cyprinus carpio

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.6 6.3
-
-
 Cyprinus carpio

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.4.6 5.5 7.5 slowly reduces its activity on the both sides of the optimum pH 6.3, at pH values of 5.5, 7.0 and 7.5, AMPD shows 13, 31 and 65% lower activity, respectively Cyprinus carpio