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Literature summary extracted from

  • Meng, G.; Fuetterer, K.
    Donor substrate recognition in the raffinose-bound E342A mutant of fructosyltransferase Bacillus subtilis levansucrase (2008), BMC Struct. Biol., 8, 16.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.10 expression in Escherichia coli DH5alpha Bacillus subtilis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.10 crystal structures of the inactive single site mutants D86A, D247A and E342A of Bacillus subtilis levansucrase and from the sucrose- and raffinose-bound complexes of the E342A mutant, minimal structural consequences of the D247A, D86A mutations Bacillus subtilis

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.10 D247A single site mutant, substitutions have little effect on the active site geometry, catalytically inactive Bacillus subtilis
2.4.1.10 D86A single site mutant, substitutions have little effect on the active site geometry, catalytically inactive Bacillus subtilis
2.4.1.10 E342A single site mutant, catalytically inactive, the E342A mutant reveals conformational flexibility of functionally relevant side chains in the vicinity of the general acid Glu342, including Arg360, a residue required for levan polymerisation Bacillus subtilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.10 extracellular
-
 Bacillus subtilis
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n Bacillus subtilis
-
 D-glucose + (2,6-beta-D-fructosyl)n+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.10 Bacillus subtilis P05655 expression in Escherichia coli DH5alpha
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.10 D-raffinose
-
 Bacillus subtilis D-fructose + melibiose
-
 ?
2.4.1.10 sucrose + (2,6-beta-D-fructosyl)n
-
 Bacillus subtilis D-glucose + (2,6-beta-D-fructosyl)n+1
-
 ?