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Literature summary extracted from

  • Liu, D.; Zhu, T.; Fan, L.; Quan, J.; Guo, H.; Ni, J.
    Identification of a novel gentisate 1,2-dioxygenase from Silicibacter pomeroyi (2007), Biotechnol. Lett., 29, 1529-1535.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.4 expression in Escherichia coli Ruegeria pomeroyi

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.4 Cu2+ 2 mM, complete inhibition Ruegeria pomeroyi
1.13.11.4 EDTA 100 mM, complete inhibition Ruegeria pomeroyi
1.13.11.4 Fe3+ 2 mM Ruegeria pomeroyi
1.13.11.4 Hg2+ 2 mM, complete inhibition Ruegeria pomeroyi
1.13.11.4 Mn2+ 2 mM Ruegeria pomeroyi
1.13.11.4 Zn2+ 2 mM Ruegeria pomeroyi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.4 0.012
-
 2,5-Dihydroxybenzoate
-
 Ruegeria pomeroyi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.4 Fe2+ required Ruegeria pomeroyi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.4 45000
-
 3 * 45000, SDS-PAGE Ruegeria pomeroyi
1.13.11.4 132000
-
 gel filtration Ruegeria pomeroyi

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.4 Ruegeria pomeroyi Q5LLB1 DSS-3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.4 recombinant enzyme Ruegeria pomeroyi

Storage Stability

EC Number Storage Stability Organism
1.13.11.4 -20°C, 1 week, purified enzyme loses 51% of its initial activity Ruegeria pomeroyi
1.13.11.4 -70°C, 1 week, purified enzyme loses 30% of its initial activity Ruegeria pomeroyi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.4 2,5-dihydroxybenzoate + O2
-
 Ruegeria pomeroyi maleylpyruvate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.4 trimer 3 * 45000, SDS-PAGE Ruegeria pomeroyi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.4 40
-
-
 Ruegeria pomeroyi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.4 8
-
-
 Ruegeria pomeroyi

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.13.11.4 Ruegeria pomeroyi isoelectric focusing 4.8 4.6