Any feedback?
Literature summary extracted from
- Encapsulation of Trigonopsis variabilis D-amino acid oxidase and fast comparison of the operational stabilities of free and immobilized preparations of the enzyme (2008), Biotechnol. Bioeng., 99, 251-260.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.4.3.3 | carrier-free enzyme is entrapped in semipermeable microcapsules produced from the polycation poly(methylene-co-guanidine) in combination with CaCl2 and the polyanions alginate and cellulose sulfate, the effectiveness of the entrapped oxidase for O2-dependent conversion of D-methionine at 25°C is 75-95% of the free enzyme preparation | Trigonopsis variabilis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.4.3.3 | poly(methylene-co-guanidine) | in the presence of poly(methylene-co-guanidine) (1.8%, w/v) the enzyme activity decreases irreversibly with a half-life time of about 1.75 min at 25°C | Trigonopsis variabilis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.4.3.3 | Trigonopsis variabilis | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.4.3.3 | D-methionine + 2,6-dichlorophenolindophenol + H2O | - |
Trigonopsis variabilis | ? | - |
? |  |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.4.3.3 | D-amino acid oxidase | - |
Trigonopsis variabilis |
| 1.4.3.3 | DAO | - |
Trigonopsis variabilis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.4.3.3 | 25 | 50 | added FAD does not stabilize DAO at and below 35°C, but it contributes up to 3.6fold extra stability to the enzyme activity at temperatures higher than 35°C, with a half-life of 60 h encapsulated DAO is 720fold more stable than the free enzyme under conditions of bubble aeration at 25°C, the soluble oxidase is stabilized by added FAD only at temperatures of 35°C or greater, at 50°C encapsulated preparations of the oxidase are much more stable than the free enzyme whose half-life is only 40 min | Trigonopsis variabilis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.4.3.3 | FAD | added FAD does not stabilize DAO at and below 35°C, but it contributes up to 3.6fold extra stability to the enzyme activity at temperatures higher than 35°C | Trigonopsis variabilis | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
