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Literature summary extracted from

  • Smutok, O.; Dmytruk, K.; Gonchar, M.; Sibirny, A.; Schuhmann, W.
    Permeabilized cells of flavocytochrome b2 over-producing recombinant yeast Hansenula polymorpha as biological recognition element in amperometric lactate biosensors (2007), Biosens. Bioelectron., 23, 599-605.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.1.2.3 analysis a L-lactate-selective microbial biosensor is developed using permeabilized cells of gene-engineered thermotolerant methylotrophic yeast Hansenula polymorpha, over-producing FCb2. The HpCYB2 gene, encoding FCb2, under the control of the strong Hansenula polymorpha alcohol oxidase promoter in the frame of a plasmid for multicopy integration is transformed to the recipient strain Hansenula polymorpha C-105 (gcr1 catX) impaired in glucose repression and devoid of catalase activity. The biosensor based on recombinant yeast cells exhibit a higher Km value (Km: 3.02 mM) and hence expanded linear range toward l-lactate as compared to a similar sensor based on the initial cells of Hansenula polymorpha C-105 (Km: 0.33 mM) Ogataea angusta

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.2.3 0.33
-
 L-lactate sensor based on the inital Hansenula polymorpha C-105 cells Ogataea angusta
1.1.2.3 3.02
-
 L-lactate biosensor based on recombinant yeast FCb2cells Ogataea angusta

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.3 Ogataea angusta
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.3 L-lactate + potassium ferricyanide
-
 Ogataea angusta pyruvate + potassium ferrocyanide + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.2.3 Fcb2
-
 Ogataea angusta
1.1.2.3 flavocytochrome b2
-
 Ogataea angusta

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.2.3 20
-
 assay at Ogataea angusta

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.2.3 7.5
-
 assay at Ogataea angusta