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Literature summary extracted from

  • Akyilmaz, E.; Yorganci, E.
    A novel biosensor based on activation effect of thiamine on the activity of pyruvate oxidase (2008), Biosens. Bioelectron., 23, 1874-1877.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.3.3 additional information pyrodoxin, nicotinic acid, and riboflavin have no activating effects Aerococcus sp.
1.2.3.3 additional information pyrodoxin, riboflavin and nicotinic acid do not play an activator role Aerococcus sp.
1.2.3.3 thiamine 0.0001 mM thiamine leads to 18.2% increase in activity Aerococcus sp.
1.2.3.3 thiamine activates the enzyme. Can be used as an activator on the biosensor responses Aerococcus sp.

Application

EC Number Application Comment Organism
1.2.3.3 additional information development of a biosensor based on POX enzyme for the investigation of the effect of thiamine (vitamin B1) molecule on the activity of the enzyme Aerococcus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.3.3 Mg2+ required for activity Aerococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
1.2.3.3 Aerococcus sp.
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.3.3 additional information cell membrane-associated electron transport system, which includes both ubiquinone-6 and cytochrome b, is the natural electron acceptor for the reduced enzyme and oxygen is the terminal electron acceptor of this system Aerococcus sp. ?
-
 ?
1.2.3.3 pyruvate + phosphate + O2
-
 Aerococcus sp. acetyl phosphate + CO2 + H2O2
-
 ?
1.2.3.3 pyruvate + phosphate + O2 + H2O
-
 Aerococcus sp. acetyl phosphate + CO2 + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.3.3 POX
-
 Aerococcus sp.
1.2.3.3 pyruvate oxidase
-
 Aerococcus sp.
1.2.3.3 pyruvate:oxygen-2-oxidoreductase (phosphorylating)
-
 Aerococcus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.3.3 35
-
-
 Aerococcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.3.3 7
-
-
 Aerococcus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.3.3 FAD
-
 Aerococcus sp.
1.2.3.3 thiamine diphosphate
-
 Aerococcus sp.