Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chakraborty, S.; Sakka, M.; Kimura, T.; Sakka, K.
    Two proteins with diaphorase activity from Clostridium thermocellum and Moorella thermoacetica (2008), Biosci. Biotechnol. Biochem., 72, 877-879.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.8.1.4 degradation shows flavin reductase activity with moderate diaphorase activity Acetivibrio thermocellus
1.8.1.4 degradation shows strong diaphorase activity Moorella thermoacetica ATCC 39073

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.4 ligated into vector pET-28a(+) and expressed in Escherichia coli BL21(DE3) as an N-terminal 6 x His-tag Acetivibrio thermocellus
1.8.1.4 ligated into vector pET-28a(+) and expressed in Escherichia coli BL21(DE3) as an N-terminal 6 x His-tag Moorella thermoacetica ATCC 39073

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.4 0.015
-
 2,6-dichlorophenolindophenol
-
 Acetivibrio thermocellus
1.8.1.4 0.03
-
 2,6-dichlorophenolindophenol
-
 Moorella thermoacetica ATCC 39073
1.8.1.4 0.32
-
 NADH
-
 Acetivibrio thermocellus
1.8.1.4 0.5
-
 NADH
-
 Moorella thermoacetica ATCC 39073

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.4 28206
-
 x * 28206, sequence analysis Acetivibrio thermocellus
1.8.1.4 28430
-
 x * 28430, sequence analysis Moorella thermoacetica ATCC 39073

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.4 Acetivibrio thermocellus
-
 ATCC 27405T
-
1.8.1.4 Moorella thermoacetica ATCC 39073
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.4 by gel filtration Acetivibrio thermocellus
1.8.1.4 by gel filtration Moorella thermoacetica ATCC 39073

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.4 2,6-dichlorophenolindophenol + NADH + H+
-
 Acetivibrio thermocellus reduced 2,6-dichlorophenolindophenol + NAD+
-
 r
1.8.1.4 2,6-dichlorophenolindophenol + NADH + H+
-
 Moorella thermoacetica ATCC 39073 reduced 2,6-dichlorophenolindophenol + NAD+
-
 r
1.8.1.4 oxidized 2,6-dichlorophenolindophenol + NADH
-
 Acetivibrio thermocellus ? + NAD+
-
 ?
1.8.1.4 oxidized 2,6-dichlorophenolindophenol + NADH
-
 Moorella thermoacetica ATCC 39073 ? + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.4 ? x * 28206, sequence analysis Acetivibrio thermocellus
1.8.1.4 ? x * 28430, sequence analysis Moorella thermoacetica ATCC 39073

Synonyms

EC Number Synonyms Comment Organism
1.8.1.4 diaphorase
-
 Acetivibrio thermocellus
1.8.1.4 diaphorase
-
 Moorella thermoacetica ATCC 39073
1.8.1.4 NADH dehydrogenase
-
 Acetivibrio thermocellus
1.8.1.4 NADH dehydrogenase
-
 Moorella thermoacetica ATCC 39073

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.1.4 50
-
-
 Acetivibrio thermocellus
1.8.1.4 50
-
-
 Moorella thermoacetica ATCC 39073

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.4 FMN tightly bound Moorella thermoacetica ATCC 39073
1.8.1.4 additional information contains neither FMN nor FAD in the molecule Acetivibrio thermocellus
1.8.1.4 NADH
-
 Acetivibrio thermocellus
1.8.1.4 NADH
-
 Moorella thermoacetica ATCC 39073
1.8.1.4 NADPH oxidizes NADPH as efficiently as NADH Acetivibrio thermocellus
1.8.1.4 NADPH oxidizes NADPH as efficiently as NADH Moorella thermoacetica ATCC 39073