EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.11.1.11 | W208F | the optical spectrum of the W208F mutant closely resembles that of wild type LmAPX at pH 7.5 in the absence of ascorbate. W208F mutant causes a spectral red shift from high spin to low spin, indicating that the mutant can react with H2O2. Cytochrome c binding affinity to the enzyme does not alter after mutation. The mutant is 1000times less active than the wild type in cytochrome c oxidation | Leishmania major |
1.11.1.11 | W208Y | mutant shows low spin hem. The mutant does not react with H2O2 | Leishmania major |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.11.1.11 | 0.017 | - |
guaiacol | 25°C, mutant enzyme W208F | Leishmania major | |
1.11.1.11 | 0.025 | - |
cytochrome c | 25°C, mutant enzyme W208F | Leishmania major | |
1.11.1.11 | 6 | - |
cytochrome c | 25°C, wild-type enzyme | Leishmania major | |
1.11.1.11 | 6.7 | - |
guaiacol | 25°C, wild-type enzyme | Leishmania major |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.11.1.11 | Leishmania major | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.11.1.11 | wild-type and mutant enzyme W208F expressed in Escherichia coli | Leishmania major |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.11.1.11 | Cytochrome c + H2O | - |
Leishmania major | ? | - |
? | |
1.11.1.11 | guaiacol + H2O2 | - |
Leishmania major | ? | - |
? | |
1.11.1.11 | L-ascorbate + H2O2 | - |
Leishmania major | dehydroascorbate + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.11.1.11 | LmAPX | - |
Leishmania major |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.11.1.11 | 0.53 | - |
ascorbate | 25°C, wild-type enzyme | Leishmania major | |
1.11.1.11 | 1.83 | - |
cytochrome c | 25°C, wild-type enzyme | Leishmania major | |
1.11.1.11 | 2.5 | - |
guaiacol | 25°C, wild-type enzyme | Leishmania major |