EC Number | Application | Comment | Organism |
---|---|---|---|
2.3.1.158 | molecular biology | the conversion of a membrane bound lipid metabolizing enzyme into a soluble and active form might be applied to other enzymes with a membrane anchor region. | Saccharomyces cerevisiae |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.158 | expression in Escherichia coli, expression in Pichia pastoris of a N-terminal deleted version of PDAT, lacking the predicted membrane-spanning region under the control of the methanol inducible alcohol oxidase promoter | Saccharomyces cerevisiae |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.3.1.158 | membrane | soluble form is produced for comparison with the native membrane bound enzyme | Saccharomyces cerevisiae | 16020 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.1.158 | 67000 | - |
SDS-PAGE, after deglycosylation | Saccharomyces cerevisiae |
2.3.1.158 | 80000 | - |
SDS-PAGE | Saccharomyces cerevisiae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.158 | Saccharomyces cerevisiae | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
2.3.1.158 | glycoprotein | extensive deglycosylation severely decreases the activity | Saccharomyces cerevisiae |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.158 | the recombinant protein by Ni2+ affinity chromatography | Saccharomyces cerevisiae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.3.1.158 | 0.00138 | - |
sn-1,2-distearoylphosphatidylethanolamine as substrate using the membrane bound enzyme | Saccharomyces cerevisiae |
2.3.1.158 | 0.001399 | - |
sn-1,2-dipalmitoylphosphatidylethanolamine as substrate using the membrane bound enzyme | Saccharomyces cerevisiae |
2.3.1.158 | 0.002453 | - |
sn-1,2-dilinolenoylphosphatidylethanolamine as substrate using the membrane bound enzyme | Saccharomyces cerevisiae |
2.3.1.158 | 0.002468 | - |
sn-1,2-dioleoylphosphatidylethanolamine as substrate using the membrane bound enzyme | Saccharomyces cerevisiae |
2.3.1.158 | 0.002597 | - |
sn-1,2-dilinoleoylphosphatidylethanolamine as substrate using the membrane bound enzyme | Saccharomyces cerevisiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.158 | rac-1,2-divernoleoylglycerol + 1,3-dioleoyl-sn-glycerol | - |
Saccharomyces cerevisiae | 1-oleoyl-sn-glycerol + rac-1,2-divernoleoyl-3-oleoylglycerol | - |
? | |
2.3.1.158 | sn-1,2-dioleoylglycerol + sn-1,2-dioleoylglycerol | - |
Saccharomyces cerevisiae | trioleoylglycerol + sn-1-oleoylglycerol | - |
? | |
2.3.1.158 | sn-1,2-dioleoylphosphatidylcholine + sn-1,2-dioleoylglycerol | - |
Saccharomyces cerevisiae | trioleoylglycerol + sn-1-oleoylphosphatidylcholine | - |
? | |
2.3.1.158 | sn-1,2-dioleoylphosphatidylcholine + sn-1-oleoylglycerol | - |
Saccharomyces cerevisiae | ? + sn-1-oleoylphosphatidylcholine | - |
? | |
2.3.1.158 | sn-1,2-dioleoylphosphatidylcholine + sn-2-oleoylglycerol | - |
Saccharomyces cerevisiae | ? + sn-1-oleoylphosphatidylcholine | - |
? | |
2.3.1.158 | sn-1,2-dioleoylphosphatidylethanolamine + sn-1,2-dioleoylglycerol | - |
Saccharomyces cerevisiae | trioleoylglycerol + 1-oleoylphosphatidylethanolamine | - |
? | |
2.3.1.158 | sn-1,3-dioleoylglycerol + sn-1,3-dioleoylglycerol | - |
Saccharomyces cerevisiae | trioleoylglycerol + sn-1-oleoylglycerol | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.158 | PDAT | - |
Saccharomyces cerevisiae |
2.3.1.158 | phospholipid:diacylglycerol acyltransferase | - |
Saccharomyces cerevisiae |
2.3.1.158 | ScPDAT | - |
Saccharomyces cerevisiae |