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Literature summary extracted from

  • Jacquot, C.; Wecksler, A.T.; McGinley, C.M.; Segraves, E.N.; Holman, T.R.; van der Donk, W.A.
    Isotope sensitive branching and kinetic isotope effects in the reaction of deuterated arachidonic acids with human 12- and 15-lipoxygenases (2008), Biochemistry, 47, 7295-7303.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.31 expression of isozyme 12-hLO as His-tagged enzyme Homo sapiens
1.13.11.33 expression of isozyme 15-hLO-1 as His-tagged enzyme, and of untagged isozyme 15-hLO-2 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.33 0.0025
-
 arachidonic acid 13,13-dideuterated arachidonic acid, pH 7.5, 25°C, recombinant isozyme 15-hLO-1 Homo sapiens
1.13.11.33 0.0028
-
 arachidonic acid 10,10,13,13-tetradeuterated arachidonic acid, pH 7.5, 25°C, recombinant isozyme 15-hLO-1 Homo sapiens
1.13.11.33 0.0034
-
 arachidonic acid 10,10-dideuterated arachidonic acid, pH 7.5, 25°C, recombinant isozyme 15-hLO-1 Homo sapiens
1.13.11.33 0.0037
-
 arachidonic acid unlabeled substrate, pH 7.5, 25°C, recombinant isozyme 15-hLO-1 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.31 Fe2+ required for the catalytic cycle of fatty acid oxidation by lipoxygenase, overview Homo sapiens
1.13.11.33 Fe2+ required for the catalytic cycle of fatty acid oxidation by lipoxygenase, overview Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.31 arachidonate + O2 Homo sapiens arachidonic acid metabolization by lipoxygenases, overview (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate
-
 ?
1.13.11.33 arachidonate + O2 Homo sapiens arachidonic acid metabolization by lipoxygenases, overview (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyeicosa-5,8,11,13-tetraenoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.31 Homo sapiens P18054 isozyme platelet 12-hLO
-
1.13.11.33 Homo sapiens
-
 two human isozymes: platelet 12-hLO, reticulocyte 15-hLO-1, and epithelial 15-hLO-2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.31 recombinant His-tagged isozyme 12-hLO by nickel affinity chromatography Homo sapiens
1.13.11.33 recombinant His-tagged isozyme 15-hLO-1 by nickel affinity chromatography, recombinant untagged isozyme 15-hLO-2 by ion exchange chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.31 blood platelet isozyme 12-hLO Homo sapiens
-
1.13.11.33 epithelium isozyme 15-hLO-2 Homo sapiens
-
1.13.11.33 reticulocyte isozyme 15-hLO-1 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.31 arachidonate + O2 arachidonic acid metabolization by lipoxygenases, overview Homo sapiens (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate
-
 ?
1.13.11.31 arachidonate + O2 regioselective oxidation is achieved through control over the position of hydrogen atom abstraction by the geometry and size of the enzyme active site and through control by the protein over the interaction of molecular oxygen with the generated delocalized substrate radical, analysis of catalytic mechanisms of lipoxygenases using 10,10-dideuterated arachidonic acid, 13,13-dideuterated arachidonic acid, and 0,10,13,13-d4-AA, overview Homo sapiens (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyeicosa-5,8,10,14-tetraenoate i.e. 12-HPETE, 12-hLO also forms small amounts of 11-HPETE, 8-HPETE, and 15-HPETE, overview ?
1.13.11.31 additional information product distributions of lipoxygenases under various conditions, overview Homo sapiens ?
-
 ?
1.13.11.33 arachidonate + O2 arachidonic acid metabolization by lipoxygenases, overview Homo sapiens (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyeicosa-5,8,11,13-tetraenoate
-
 ?
1.13.11.33 arachidonate + O2 regioselective oxidation is achieved through control over the position of hydrogen atom abstraction by the geometry and size of the enzyme active site and through control by the protein over the interaction of molecular oxygen with the generated delocalized substrate radical, analysis of catalytic mechanisms of lipoxygenases using 10,10-dideuterated arachidonic acid, 13,13-dideuterated arachidonic acid, and 0,10,13,13-d4-AA, overview Homo sapiens (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyeicosa-5,8,11,13-tetraenoate isozymes 15-hLO-1 and 15-hLO-2 also form small amounts of 11-HPETE, 8-HPETE, and 12-HPETE, the rate of 8/12-HPETE production by isozyme 15-hLO-1 with 13,13-dideuterated arachidonic acid as substrate is exeptionally high with 75% of overall production, overview ?
1.13.11.33 additional information product distributions of lipoxygenases under various conditions, overview Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.31 12-lipoxygenase
-
 Homo sapiens
1.13.11.33 15-lipoxygenase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.31 25
-
 assay at Homo sapiens
1.13.11.33 25
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.33 1
-
 arachidonic acid 10,10,13,13-tetradeuterated arachidonic acid, pH 7.5, 25°C, recombinant isozyme 15-hLO-1 Homo sapiens
1.13.11.33 2.49
-
 arachidonic acid 13,13-dideuterated arachidonic acid, pH 7.5, 25°C, recombinant isozyme 15-hLO-1 Homo sapiens
1.13.11.33 9.26
-
 arachidonic acid 10,10-dideuterated arachidonic acid, pH 7.5, 25°C, recombinant isozyme 15-hLO-1 Homo sapiens
1.13.11.33 9.93
-
 arachidonic acid unlabeled substrate, pH 7.5, 25°C, recombinant isozyme 15-hLO-1 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.31 7.5
-
 assay at Homo sapiens
1.13.11.33 7.5
-
 assay at Homo sapiens