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Literature summary extracted from

  • Szabo, J.; Varga, A.; Flachner, B.; Konarev, P.V.; Svergun, D.I.; Zavodszky, P.; Vas, M.
    Communication between the nucleotide site and the main molecular hinge of 3-phosphoglycerate kinase (2008), Biochemistry, 47, 6735-6744.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.7.2.3 E343A kcat for reaction with ATP + 3-phosphoglycerate is 58fold lower than wild-type value, kcat for reaction with ADP + 3-phospho-D-glyceroyl phosphate is 31fold lower than wild-type value Homo sapiens
2.7.2.3 K215A kcat for reaction with ATP + 3-phosphoglycerate is 1735fold lower than wild-type value, kcat for reaction with ADP + 3-phospho-D-glyceroyl phosphate is 3376fold lower than wild-type value Homo sapiens
2.7.2.3 K219A kcat for reaction with ATP + 3-phosphoglycerate is 925fold lower than wild-type value, kcat for reaction with ADP + 3-phospho-D-glyceroyl phosphate is 502fold lower than wild-type value Homo sapiens
2.7.2.3 N336A kcat for reaction with ATP + 3-phosphoglycerate is 139fold lower than wild-type value, kcat for reaction with ADP + 3-phospho-D-glyceroyl phosphate is 113fold lower than wild-type value Homo sapiens
2.7.2.3 R38A kcat for reaction with ATP + 3-phosphoglycerate is 1851fold lower than wild-type value, kcat for reaction with ADP + 3-phospho-D-glyceroyl phosphate is 439fold lower than wild-type value Homo sapiens
2.7.2.3 T375A kcat for reaction with ATP + 3-phosphoglycerate is 6.7fold lower than wild-type value, kcat for reaction with ADP + 3-phospho-D-glyceroyl phosphate is 3.9fold lower than wild-type value Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.3 diphosphate
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.3 0.0013
-
 3-phospho-D-glyceroyl phosphate mutant enzyme E343A Homo sapiens
2.7.2.3 0.0022
-
 3-phospho-D-glyceroyl phosphate mutant enzyme N336A Homo sapiens
2.7.2.3 0.003
-
 3-phospho-D-glyceroyl phosphate mutant enzyme T375A Homo sapiens
2.7.2.3 0.0038
-
 3-phospho-D-glyceroyl phosphate mutant enzyme K125A Homo sapiens
2.7.2.3 0.005
-
 3-phospho-D-glyceroyl phosphate wild-type enzyme Homo sapiens
2.7.2.3 0.0077
-
 3-phospho-D-glyceroyl phosphate mutant enzyme k219A Homo sapiens
2.7.2.3 0.1
-
 3-phospho-D-glycerate wild-type enzyme Homo sapiens
2.7.2.3 0.1
-
 ADP mutant enzyme R38A Homo sapiens
2.7.2.3 0.11
-
 ATP wild-type enzyme Homo sapiens
2.7.2.3 0.12
-
 ADP wild-type enzyme Homo sapiens
2.7.2.3 0.15
-
 ADP mutant enzyme K125A Homo sapiens
2.7.2.3 0.29
-
 3-phospho-D-glyceroyl phosphate mutant enzyme R38A Homo sapiens
2.7.2.3 0.31
-
 3-phospho-D-glycerate mutant enzyme E343A Homo sapiens
2.7.2.3 0.41
-
 3-phospho-D-glycerate mutant enzyme K125A Homo sapiens
2.7.2.3 0.42
-
 ADP mutant enzyme N336A Homo sapiens
2.7.2.3 0.54
-
 3-phospho-D-glycerate mutant enzyme N336A Homo sapiens
2.7.2.3 0.63
-
 3-phospho-D-glycerate mutant enzyme T375A Homo sapiens
2.7.2.3 0.91
-
 ADP mutant enzyme E343A Homo sapiens
2.7.2.3 0.96
-
 ATP mutant enzyme R38A Homo sapiens
2.7.2.3 1.04
-
 ATP mutant enzyme T375A Homo sapiens
2.7.2.3 1.12
-
 ADP mutant enzyme T375A Homo sapiens
2.7.2.3 1.34
-
 ATP mutant enzyme N336A Homo sapiens
2.7.2.3 1.42
-
 ADP mutant enzyme k219A Homo sapiens
2.7.2.3 1.69
-
 ATP mutant enzyme E343A Homo sapiens
2.7.2.3 1.72
-
 3-phospho-D-glycerate mutant enzyme k219A Homo sapiens
2.7.2.3 2.47
-
 ATP mutant enzyme K125A Homo sapiens
2.7.2.3 4.43
-
 ATP mutant enzyme k219A Homo sapiens
2.7.2.3 4.5
-
 3-phospho-D-glycerate mutant enzyme R38A Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.3 Homo sapiens P00558
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.3 ADP + 3-phospho-D-glyceroyl phosphate catalytic residue R38, which also binds the substrate 3-phosphoglycerate, is essential in inducing domain closure. Similarly, residues K219, N336, and E343 which interact with the nucleotide substrates are involved in the process of domain closure. The other catalytic residue, K215, covers a large distance during catalysis but has no direct role in domain closure Homo sapiens ATP + 3-phospho-D-glycerate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.2.3 3-phosphoglycerate kinase
-
 Homo sapiens
2.7.2.3 hPGK
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.2.3 0.45
-
 ATP mutant enzyme R38A Homo sapiens
2.7.2.3 0.45
-
 3-phospho-D-glycerate mutant enzyme R38A Homo sapiens
2.7.2.3 0.48
-
 ATP mutant enzyme K125A Homo sapiens
2.7.2.3 0.48
-
 3-phospho-D-glycerate mutant enzyme K125A Homo sapiens
2.7.2.3 0.78
-
 ADP mutant enzyme K125A Homo sapiens
2.7.2.3 0.78
-
 3-phospho-D-glyceroyl phosphate mutant enzyme K125A Homo sapiens
2.7.2.3 0.9
-
 ATP mutant enzyme k219A Homo sapiens
2.7.2.3 0.9
-
 3-phospho-D-glycerate mutant enzyme k219A Homo sapiens
2.7.2.3 5.25
-
 ADP mutant enzyme k219A Homo sapiens
2.7.2.3 5.25
-
 3-phospho-D-glyceroyl phosphate mutant enzyme k219A Homo sapiens
2.7.2.3 6
-
 ATP mutant enzyme N336A Homo sapiens
2.7.2.3 6
-
 3-phospho-D-glycerate mutant enzyme N336A Homo sapiens
2.7.2.3 6
-
 ADP mutant enzyme R38A Homo sapiens
2.7.2.3 6
-
 3-phospho-D-glyceroyl phosphate mutant enzyme R38A Homo sapiens
2.7.2.3 14.3
-
 ATP mutant enzyme E343A Homo sapiens
2.7.2.3 14.3
-
 3-phospho-D-glycerate mutant enzyme E343A Homo sapiens
2.7.2.3 23.2
-
 3-phospho-D-glyceroyl phosphate mutant enzyme N336A Homo sapiens
2.7.2.3 23.3
-
 ADP mutant enzyme N336A Homo sapiens
2.7.2.3 84
-
 ADP mutant enzyme E343A Homo sapiens
2.7.2.3 84
-
 3-phospho-D-glyceroyl phosphate mutant enzyme E343A Homo sapiens
2.7.2.3 125
-
 ATP mutant enzyme T375A Homo sapiens
2.7.2.3 125
-
 3-phospho-D-glycerate mutant enzyme T375A Homo sapiens
2.7.2.3 675
-
 ADP mutant enzyme T375A Homo sapiens
2.7.2.3 675
-
 3-phospho-D-glyceroyl phosphate mutant enzyme T375A Homo sapiens
2.7.2.3 833
-
 ATP wild-type enzyme Homo sapiens
2.7.2.3 833
-
 3-phospho-D-glycerate wild-type enzyme Homo sapiens
2.7.2.3 2633
-
 ADP wild-type enzyme Homo sapiens
2.7.2.3 2633
-
 3-phospho-D-glyceroyl phosphate wild-type enzyme Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.2.3 0.09
-
 diphosphate mutant enzyme N336A Homo sapiens
2.7.2.3 0.13
-
 diphosphate mutant enzyme R38A Homo sapiens
2.7.2.3 0.14
-
 diphosphate mutant enzyme E343A Homo sapiens
2.7.2.3 0.22
-
 diphosphate wild-type enzyme Homo sapiens
2.7.2.3 0.51
-
 diphosphate mutant enzyme K125A Homo sapiens
2.7.2.3 0.52
-
 diphosphate mutant enzyme k219A Homo sapiens
2.7.2.3 1.9
-
 diphosphate mutant enzyme T375A Homo sapiens