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Literature summary extracted from

  • Murray, M.S.; Holmes, R.P.; Lowther, W.T.
    Active site and loop 4 movements within human glycolate oxidase implications for substrate specificity and drug design (2008), Biochemistry, 47, 2439-2449.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.15 expressed in Escherichia coli as a His-tagged fusion protein Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.3.15 structures of recombinant GO complexed with sulfate, glyoxylate, and 4-carboxy-5-dodecylsulfanyl-1,2,3-triazole (CDST), determined by X-ray crystallography are reported. A loop region (loop 4), is completely visible when the GO active site contains a small ligand. Lack of electron density for this loop in the GOCDST complex, mimicking a large substrate, indicates that a disordered to ordered transition occurs with substrate binding. Conformational flexibility of Trp110 is responsible for enabling GO to react with R-hydroxy acids. Movement of Trp110 disrupts a hydrogen-bonding network between Trp110, Leu191, Tyr134, and Tyr208. This loss indicates that active site movements are directly linked to changes in the conformation of loop 4 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.15 4-carboxy-5-dodecylsulfanyl-1,2,3-triazole
-
 Homo sapiens
1.1.3.15 glyoxylate substrate inhibition at concentrations above 4 mM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.15 0.04
-
 2-oxo-octanoate
-
 Homo sapiens
1.1.3.15 0.141
-
 glycolate
-
 Homo sapiens
1.1.3.15 2.2
-
 glyoxylate
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.15 Homo sapiens Q9UJM8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.15 using a Ni-NTA-column, gel filtration and ion-exchange chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.15 2-hydroxyoctanoate + 2,6-dichlorophenolindophenol
-
 Homo sapiens 2-oxo-octanoate + reduced 2,6-dichlorophenolindophenol
-
 r
1.1.3.15 glycolate + 2,6-dichlorophenolindophenol
-
 Homo sapiens glyoxylate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.3.15 glyoxylate + 2,6-dichlorophenolindophenol
-
 Homo sapiens ? + reduced 2,6-dichlorophenolindophenol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.3.15 glycolate oxidase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.15 37
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.15 0.08
-
 2-oxo-octanoate
-
 Homo sapiens
1.1.3.15 0.7
-
 glyoxylate
-
 Homo sapiens
1.1.3.15 4.1
-
 glycolate
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.15 7.5
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.15 FMN
-
 Homo sapiens