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Literature summary extracted from

  • Abreu, I.A.; Hearn, A.; An, H.; Nick, H.S.; Silverman, D.N.; Cabelli, D.E.
    The kinetic mechanism of manganese-containing superoxide dismutase from Deinococcus radiodurans: a specialized enzyme for the elimination of high superoxide concentrations (2008), Biochemistry, 47, 2350-2356.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.15.1.1 from genomic DNA, expression in Escherichia coli strain QC774 Deinococcus radiodurans

Protein Variants

EC Number Protein Variants Comment Organism
1.15.1.1 Y34F the mutant shows metalcofactor kinetics similar to the human not the Deinococcus radiodurans Mn-SOD, formation of human-like Mn3+SOD and human-like Mn3+SOD-O2 - adduct, overview Deinococcus radiodurans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.15.1.1 additional information
-
 additional information rate constants and kinetic mechanism Deinococcus radiodurans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.1 Manganese when the Deinococcus radiodurans Mn2+SOD reacts with a substoichiometric amount of superoxide, Deinococcus radiodurans Mn3+SOD is produced Deinococcus radiodurans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.15.1.1 22000
-
 x * 22000, recombinant Mn-SOD, SDS-PAGE Deinococcus radiodurans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.15.1.1 O2.- + H+ Deinococcus radiodurans Deinococcus radiodurans Mn-SOD is most effective at high superoxide fluxes found under conditions of high radioactivity compared to te enzyme of Escherichia coli and Homo sapiens O2 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.1 Deinococcus radiodurans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.15.1.1 recombinant Mn-SOD from Escherichia coli strain QC774 by anion exchange chromatography Deinococcus radiodurans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.15.1.1 O2.- + H+
-
 Deinococcus radiodurans O2 + H2O2
-
 ?
1.15.1.1 O2.- + H+ Deinococcus radiodurans Mn-SOD is most effective at high superoxide fluxes found under conditions of high radioactivity compared to te enzyme of Escherichia coli and Homo sapiens Deinococcus radiodurans O2 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.15.1.1 ? x * 22000, recombinant Mn-SOD, SDS-PAGE Deinococcus radiodurans

Synonyms

EC Number Synonyms Comment Organism
1.15.1.1 manganese-containing superoxide dismutase
-
 Deinococcus radiodurans
1.15.1.1 MnSOD
-
 Deinococcus radiodurans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.15.1.1 25
-
 assay at Deinococcus radiodurans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.15.1.1 7
-
 assay at Deinococcus radiodurans