Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Forbes, S.P.; Druhan, L.J.; Guzman, J.E.; Parinandi, N.; Zhang, L.; Green-Church, K.B.; Cardounel, A.J.
    Mechanism of 4-HNE mediated inhibition of hDDAH-1: implications in no regulation (2008), Biochemistry, 47, 1819-1826.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.3.18 expressed in Escherichia coli BL21star cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.3.18 4-hydroxy-nonenal dose-dependently inhibits DDAH activity with 15% inhibition at 0.01 mM and complete inhibition at 0.5 mM Homo sapiens
3.5.3.18 H2O2 43% inhibition at 1 mM Homo sapiens
3.5.3.18 additional information exposure to pathophysiological levels of reactive oxygen has little to no effect on the activity of the enzyme Homo sapiens
3.5.3.18 OH radical 47% inhibition at 1 mM Homo sapiens
3.5.3.18 peroxynitrite inhibition of 15% after exposure to peroxynitrite at concentrations ranging from 0.01-0.1 mM, 25% inhibition at 1 mM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.18 0.536
-
 NG-methyl-L-arginine at 37°C Homo sapiens
3.5.3.18 0.687
-
 NG,NG-dimethyl-L-arginine at 37°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.3.18 Zn2+ Zn2+-containing enzyme Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.18 37000
-
 DDAH-1, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.18 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.18 HisTrap column chromatography and HiTrap Q Sepharose column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.18 additional information incapable of hydrolyzing peptide incorporated methylarginines Homo sapiens ?
-
 ?
3.5.3.18 NG,NG-dimethyl-L-arginine + H2O
-
 Homo sapiens dimethylamine + L-citrulline
-
 ?
3.5.3.18 NG-methyl arginine + H2O
-
 Homo sapiens L-citrulline + methylamine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.3.18 DDAH-1
-
 Homo sapiens
3.5.3.18 dimethylarginine dimethylamino-hydrolase
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.18 8.5
-
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.3.18 5.5 9.5
-
 Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.5.3.18 0.05
-
 at 37°C Homo sapiens 4-hydroxy-nonenal