Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bennett, J.P.; Whittingham, J.L.; Brzozowski, A.M.; Leonard, P.M.; Grogan, G.
    Structural characterization of a beta-diketone hydrolase from the cyanobacterium Anabaena sp. PCC 7120 in native and product-bound forms, a coenzyme A-independent member of the crotonase suprafamily (2007), Biochemistry, 46, 137-144.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.7.1.7 into the pETYSBLIC vector for expression in Escherichia coli BL21DE3 cells Nostoc sp. PCC 7120 = FACHB-418

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.7.1.7 the structure of beta-diketone hydrolase is determined to a resolution of 1.5 A in both native and ligand-bound forms Nostoc sp. PCC 7120 = FACHB-418

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.7.1.7 0.0000044
-
 bicyclo[2.2.2]octane-2,6-dione
-
 Nostoc sp. PCC 7120 = FACHB-418

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.7.1.7 28800
-
 predicted molecular mass Nostoc sp. PCC 7120 = FACHB-418
3.7.1.7 30000
-
 determined by SDS-PAGE Nostoc sp. PCC 7120 = FACHB-418

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.7.1.7 nonane-4,6-dione + H2O Nostoc sp. PCC 7120 = FACHB-418
-
 pentan-2-one + butanoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.7.1.7 Nostoc sp. PCC 7120 = FACHB-418 Q8YNV6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.7.1.7 using a nickel affinity HiTRAP agarose column and a S200 16/60 Sephadex column Nostoc sp. PCC 7120 = FACHB-418

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.7.1.7 bicyclo[2.2.2]octane-2,6-dione + H2O desymmetrization of bicyclo[2.2.2]octane-2,6-dione Nostoc sp. PCC 7120 = FACHB-418 [(S)-3-oxocyclohexyl]acetic acid
-
 ?
3.7.1.7 nonane-4,6-dione + H2O
-
 Nostoc sp. PCC 7120 = FACHB-418 pentan-2-one + butanoate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.7.1.7 homohexamer
-
 Nostoc sp. PCC 7120 = FACHB-418

Synonyms

EC Number Synonyms Comment Organism
3.7.1.7 ABDH
-
 Nostoc sp. PCC 7120 = FACHB-418
3.7.1.7 Alr4455 protein
-
 Nostoc sp. PCC 7120 = FACHB-418
3.7.1.7 beta-diketone hydrolase
-
 Nostoc sp. PCC 7120 = FACHB-418

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.7.1.7 0.16
-
 bicyclo[2.2.2]octane-2,6-dione
-
 Nostoc sp. PCC 7120 = FACHB-418

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.7.1.7 7
-
 retro-Claisenase activity assay Nostoc sp. PCC 7120 = FACHB-418