BRENDA - Enzyme Database show

Biochemical and structural characterization of Pseudomonas aeruginosa Bfd and FPR: ferredoxin NADP+ reductase and not ferredoxin is the redox partner of heme oxygenase under iron-starvation conditions

Wang, A.; Zeng, Y.; Han, H.; Weeratunga, S.; Morgan, B.N.; Moenne-Loccoz, P.; Schoenbrunn, E.; Rivera, M.; Biochemistry 46, 12198-12211 (2007)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.18.1.2
expressed in Escherichia coli ArcticExpress RIL cells
Pseudomonas aeruginosa
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.18.1.2
Pseudomonas aeruginosa
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.18.1.2
Q-Sepharose Fast Flow column chromatography and Sephadex G-50 gel filtration
Pseudomonas aeruginosa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.18.1.2
additional information
FPR supports the efficient degradation of heme by heme oxygenase
685098
Pseudomonas aeruginosa
?
-
-
-
-
1.18.1.2
oxidized ferredoxin + NADH
very slow and inefficient reaction
685098
Pseudomonas aeruginosa
reduced ferredoxin + NAD+
-
-
-
?
1.18.1.2
reduced ferredoxin + NADP+
NADPH is the reducing agent of FPR in vivo
685098
Pseudomonas aeruginosa
oxidized ferredoxin + NADPH
-
-
-
r
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.18.1.2
FAD
FAD is reduced rapidly and completely when the enzyme reacts with NADPH, while in contrast, addition of NADH results in very slow and inefficient reduction
Pseudomonas aeruginosa
1.18.1.2
NADP+
-
Pseudomonas aeruginosa
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.18.1.2
expressed in Escherichia coli ArcticExpress RIL cells
Pseudomonas aeruginosa
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.18.1.2
FAD
FAD is reduced rapidly and completely when the enzyme reacts with NADPH, while in contrast, addition of NADH results in very slow and inefficient reduction
Pseudomonas aeruginosa
1.18.1.2
NADP+
-
Pseudomonas aeruginosa
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.18.1.2
Q-Sepharose Fast Flow column chromatography and Sephadex G-50 gel filtration
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.18.1.2
additional information
FPR supports the efficient degradation of heme by heme oxygenase
685098
Pseudomonas aeruginosa
?
-
-
-
-
1.18.1.2
oxidized ferredoxin + NADH
very slow and inefficient reaction
685098
Pseudomonas aeruginosa
reduced ferredoxin + NAD+
-
-
-
?
1.18.1.2
reduced ferredoxin + NADP+
NADPH is the reducing agent of FPR in vivo
685098
Pseudomonas aeruginosa
oxidized ferredoxin + NADPH
-
-
-
r