Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hoeffken, H.W.; Duong, M.; Friedrich, T.; Breuer, M.; Hauer, B.; Reinhardt, R.; Rabus, R.; Heider, J.
    Crystal structure and enzyme kinetics of the (S)-specific 1-phenylethanol dehydrogenase of the denitrifying bacterium strain EbN1 (2006), Biochemistry, 45, 82-93.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.B3 expression in Escherichia coli uncultured bacterium
1.1.1.311 overexpressed in Escherichia coli Azoarcus sp.

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.B3 apo-enzyme and bound to NAD+, at a resolution of 2.1 and 2.4 A, respectively. Enzyme is a tetamer with two types of hydrophobic interfaces. NAD+-binding is associated with a conformational shift of the substrate binding loop from a crystallographically unordered open to a more ordered closed form uncultured bacterium
1.1.1.311 sitting drop method in a buffer containing 12-18% PEG 8000, 0.05 M potassium phosphate, pH 7.5 and 3% 2-methyl-2,4-pentanediol. The structure of the apo-form of the enzyme is solved with a resolution at 2.1 A. The enzyme soaked with NAD+ and the inhibitor (R)-1-phenylethanol forms a different crystal form containing two subunits in the asymmetric unit Azoarcus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.B3 (R)-1-phenylethanol
-
 uncultured bacterium
1.1.1.B3 (S)-1-phenylethanol strong substrate inhibition, 50% of maximum activity at 0.1 mM uncultured bacterium
1.1.1.B3 acetophenone inhibitory above 0.02 mM, 75% of maximum activity at 1 mM uncultured bacterium
1.1.1.311 (R)-1-phenylethanol 0.05 mM, 50% inhibition of acetophenone reduction activity Azoarcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.B3 additional information
-
 additional information enzyme exhibits positive cooperativity in both forward and reverse direction uncultured bacterium
1.1.1.B3 0.0055
-
 (S)-1-phenylethanol pH 7.8, 25°C uncultured bacterium
1.1.1.B3 0.0113
-
 acetophenone pH 7.8, 25°C uncultured bacterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.B3 27000
-
 4 * 27000, SDS-PAGE and crystallization data, enzyme is a tetramer with two types of hydrophobic interfaces uncultured bacterium
1.1.1.B3 59000
-
 gel filtration uncultured bacterium
1.1.1.B3 93000
-
 PAGE uncultured bacterium
1.1.1.311 27000
-
 4 * 27000 Azoarcus sp.
1.1.1.311 93000
-
 gel filtration Azoarcus sp.

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.B3 uncultured bacterium
-
-
-
1.1.1.B3 uncultured bacterium EbN1
-
-
-
1.1.1.311 Azoarcus sp. Q5P5I4
-
-
1.1.1.311 Azoarcus sp. EbN1 Q5P5I4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.B3 recombinant protein uncultured bacterium
1.1.1.311
-
 Azoarcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.B3 (S)-1-phenylethanol + NAD+
-
 uncultured bacterium acetophenone + NADH + H+
-
 r
1.1.1.B3 (S)-1-phenylethanol + NAD+
-
 uncultured bacterium EbN1 acetophenone + NADH + H+
-
 r
1.1.1.B3 acetophenone + NADH + H+
-
 uncultured bacterium (S)-1-phenylethanol + NAD+
-
 r
1.1.1.B3 acetophenone + NADH + H+
-
 uncultured bacterium EbN1 (S)-1-phenylethanol + NAD+
-
 r
1.1.1.311 (S)-1-phenylethanol + NAD+
-
 Azoarcus sp. acetophenone + NADH + H+
-
 r
1.1.1.311 (S)-1-phenylethanol + NAD+
-
 Azoarcus sp. EbN1 acetophenone + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.B3 tetramer 4 * 27000, SDS-PAGE and crystallization data, enzyme is a tetramer with two types of hydrophobic interfaces uncultured bacterium
1.1.1.311 tetramer 4 * 27000 Azoarcus sp.

Synonyms

EC Number Synonyms Comment Organism
1.1.1.B3 (S)-1-phenylethanol dehydrogenase
-
 uncultured bacterium
1.1.1.B3 PED
-
 uncultured bacterium
1.1.1.311 PED
-
 Azoarcus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.311 25
-
 assay at Azoarcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.311 7.8
-
 assay at Azoarcus sp.