EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.4 | expressed in Escherichia coli as a GST-fusion protein | Aspergillus fumigatus |
2.3.1.4 | expressed in Escherichia coli as a His-tagged-fusion protein | Homo sapiens |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.3.1.4 | crystal structures of human and Aspergillus fumigatus GNA1 are determined: structural differences between the two enzymes are mostly located to the sugar-binding site, whereas the AcCoA-binding site is more conserved. These changes affect not only the electrostatics, but also reveal a more spacious sugar binding site in the Aspergillus fumigatus GNA1 enzyme, whereas large side chains at these positions create a tighter pocket in the HsGNA1 enzyme | Aspergillus fumigatus |
2.3.1.4 | crystal structures of human and Aspergillus fumigatus GNA1 are determined: structural differences between the two enzymes are mostly located to the sugar-binding site, whereas the AcCoA-binding site is more conserved. These changes affect not only the electrostatics, but also reveal a more spacious sugar binding site in the Aspergillus fumigatus GNA1 enzyme, whereas large side chains at these positions create a tighter pocket in the HsGNA1 enzyme | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.4 | E185Y | Km (mM): 0.03 (D-glucosamine 6-phosphate), 0.04 (acetyl-CoA), kcat (1/sec): 1.34 (D-glucosamine 6-phosphate) | Aspergillus fumigatus |
2.3.1.4 | G183E | Km (mM): 0.056 (D-glucosamine 6-phosphate), 0.1 (acetyl-CoA), kcat (1/sec): 0.34 (D-glucosamine 6-phosphate) | Aspergillus fumigatus |
2.3.1.4 | additional information | mutation of the four nonconserved residues (V125, G183, E185 and Y189) lining the sugar-binding pocket suggests that they contribute to the kinetic differences between GNA1 from Aspergillus fumigatus and GNA1 from Homo sapiens | Aspergillus fumigatus |
2.3.1.4 | V125R | Km (mM): 0.6 (D-glucosamine 6-phosphate), 0.2 (acetyl-CoA), kcat (1/sec): 0.12 (D-glucosamine 6-phosphate) | Aspergillus fumigatus |
2.3.1.4 | Y189R | Km (mM): 0.056 (D-glucosamine 6-phosphate), 0.086 (acetyl-CoA), kcat (1/sec): 1.7 (D-glucosamine 6-phosphate) | Aspergillus fumigatus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.4 | 0.026 | - |
acetyl-CoA | wild-type | Homo sapiens | |
2.3.1.4 | 0.03 | - |
D-glucosamine 6-phosphate | mutant E185Y | Aspergillus fumigatus | |
2.3.1.4 | 0.04 | - |
acetyl-CoA | wild-type | Aspergillus fumigatus | |
2.3.1.4 | 0.04 | - |
acetyl-CoA | mutant E185Y | Aspergillus fumigatus | |
2.3.1.4 | 0.056 | - |
D-glucosamine 6-phosphate | mutant G183E | Aspergillus fumigatus | |
2.3.1.4 | 0.056 | - |
D-glucosamine 6-phosphate | mutant Y189R | Aspergillus fumigatus | |
2.3.1.4 | 0.071 | - |
D-glucosamine 6-phosphate | wild-type | Aspergillus fumigatus | |
2.3.1.4 | 0.086 | - |
acetyl-CoA | mutant Y189R | Aspergillus fumigatus | |
2.3.1.4 | 0.097 | - |
D-glucosamine 6-phosphate | wild-type | Homo sapiens | |
2.3.1.4 | 0.1 | - |
acetyl-CoA | mutant G183E | Aspergillus fumigatus | |
2.3.1.4 | 0.2 | - |
acetyl-CoA | mutant V125R | Aspergillus fumigatus | |
2.3.1.4 | 0.6 | - |
D-glucosamine 6-phosphate | mutant V125R | Aspergillus fumigatus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.4 | Aspergillus fumigatus | - |
- |
- |
2.3.1.4 | Homo sapiens | Q96EK6 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.4 | using affinity, ion-exchange and size-exclusion chromatography: yield 25 mg of pure HsGNA1 per litre of bacterial culture | Homo sapiens |
2.3.1.4 | using affinity, ion-exchange and size-exclusion chromatography: yield 4 mg of pure AfGNA1 per litre of bacterial culture | Aspergillus fumigatus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.3.1.4 | 25 | - |
- |
Homo sapiens |
2.3.1.4 | 107 | - |
- |
Aspergillus fumigatus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.4 | acetyl-CoA + D-glucosamine 6-phosphate | - |
Aspergillus fumigatus | CoA + N-acetyl-D-glucosamine 6-phosphate | - |
? | |
2.3.1.4 | acetyl-CoA + D-glucosamine 6-phosphate | - |
Homo sapiens | CoA + N-acetyl-D-glucosamine 6-phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.1.4 | homodimer | crystal structure | Aspergillus fumigatus |
2.3.1.4 | homodimer | crystal structure | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.4 | AfGNA1 | - |
Aspergillus fumigatus |
2.3.1.4 | glucosamine-6-phosphate N-acetyltransferase | - |
Aspergillus fumigatus |
2.3.1.4 | glucosamine-6-phosphate N-acetyltransferase | - |
Homo sapiens |
2.3.1.4 | HsGNA1 | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.4 | additional information | - |
assay carried out at room temperature | Aspergillus fumigatus |
2.3.1.4 | additional information | - |
assay carried out at room temperature | Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.4 | 0.12 | - |
D-glucosamine 6-phosphate | mutant V125R | Aspergillus fumigatus | |
2.3.1.4 | 0.34 | - |
D-glucosamine 6-phosphate | mutant G183E | Aspergillus fumigatus | |
2.3.1.4 | 1.34 | - |
D-glucosamine 6-phosphate | mutant E185Y | Aspergillus fumigatus | |
2.3.1.4 | 1.7 | - |
D-glucosamine 6-phosphate | mutant Y189R | Aspergillus fumigatus | |
2.3.1.4 | 9 | - |
D-glucosamine 6-phosphate | wild-type | Homo sapiens | |
2.3.1.4 | 38 | - |
D-glucosamine 6-phosphate | wild-type | Aspergillus fumigatus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.4 | 7.5 | - |
assay at | Aspergillus fumigatus |
2.3.1.4 | 7.5 | - |
assay at | Homo sapiens |