BRENDA - Enzyme Database show

A lipoxygenase with linoleate diol synthase activity from Nostoc sp. PCC 7120

Lang, I.; Goebel, C.; Porzel, A.; Heilmann, I.; Feussner, I.; Biochem. J. 410, 347-357 (2008)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.13.11.61
expression of separate domains of NspLOX and the entire protein in Escherichia coli
Nostoc sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.13.11.61
70900
-
x * 70900, calculated from sequence
Nostoc sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.13.11.61
alpha-linolenate + O2
Nostoc sp.
i.e. (9Z,12Z,15Z)-octadeca-9,12,15-trienoate
(9R,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate
-
-
?
1.13.11.61
linoleate + O2
Nostoc sp.
the enzyme is involved in oxylipin biosyntesis
(9R,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.13.11.B1
Nostoc sp.
Q8YK97
strain PCC 7120
-
1.13.11.61
Nostoc sp.
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.11.B1
alpha-linolenic acid + O2
preferred substrate
684995
Nostoc sp.
(10E,12E,14E)-9,16-dihydroxy-10,12,14-octadecatrienoic acid
-
-
-
?
1.13.11.B1
linoleic acid + O2
-
684995
Nostoc sp.
(10E,12E)-9,14-dihydroxy-10,12-octadecadienoic acid
-
-
-
?
1.13.11.61
alpha-linolenate + O2
i.e. (9Z,12Z,15Z)-octadeca-9,12,15-trienoate
684995
Nostoc sp.
(9R,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate
-
-
-
?
1.13.11.61
alpha-linolenate + O2
i.e. (9Z,12Z,15Z)-octadeca-9,12,15-trienoate. Preferred substrate
684995
Nostoc sp.
(9R,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate
-
-
-
?
1.13.11.61
linoleate + O2
the enzyme is involved in oxylipin biosyntesis
684995
Nostoc sp.
(9R,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate
-
-
-
?
1.13.11.61
linoleate + O2
i.e. (9Z,12Z)-octadeca-9,12-dienoate, the C-terminal portion of NspLOX is identified as a linoleate linoleate (9R)-lipoxygenase. 42% of the activity with alpha-linolenic acid
684995
Nostoc sp.
(9R,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.13.11.61
?
x * 70900, calculated from sequence
Nostoc sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.11.B1
7
10
-
Nostoc sp.
1.13.11.61
7
10
broad pH-optimum
Nostoc sp.
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.13.11.61
expression of separate domains of NspLOX and the entire protein in Escherichia coli
Nostoc sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.13.11.61
70900
-
x * 70900, calculated from sequence
Nostoc sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.13.11.61
alpha-linolenate + O2
Nostoc sp.
i.e. (9Z,12Z,15Z)-octadeca-9,12,15-trienoate
(9R,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate
-
-
?
1.13.11.61
linoleate + O2
Nostoc sp.
the enzyme is involved in oxylipin biosyntesis
(9R,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.11.B1
alpha-linolenic acid + O2
preferred substrate
684995
Nostoc sp.
(10E,12E,14E)-9,16-dihydroxy-10,12,14-octadecatrienoic acid
-
-
-
?
1.13.11.B1
linoleic acid + O2
-
684995
Nostoc sp.
(10E,12E)-9,14-dihydroxy-10,12-octadecadienoic acid
-
-
-
?
1.13.11.61
alpha-linolenate + O2
i.e. (9Z,12Z,15Z)-octadeca-9,12,15-trienoate
684995
Nostoc sp.
(9R,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate
-
-
-
?
1.13.11.61
alpha-linolenate + O2
i.e. (9Z,12Z,15Z)-octadeca-9,12,15-trienoate. Preferred substrate
684995
Nostoc sp.
(9R,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate
-
-
-
?
1.13.11.61
linoleate + O2
the enzyme is involved in oxylipin biosyntesis
684995
Nostoc sp.
(9R,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate
-
-
-
?
1.13.11.61
linoleate + O2
i.e. (9Z,12Z)-octadeca-9,12-dienoate, the C-terminal portion of NspLOX is identified as a linoleate linoleate (9R)-lipoxygenase. 42% of the activity with alpha-linolenic acid
684995
Nostoc sp.
(9R,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.13.11.61
?
x * 70900, calculated from sequence
Nostoc sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.11.B1
7
10
-
Nostoc sp.
1.13.11.61
7
10
broad pH-optimum
Nostoc sp.