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Literature summary extracted from

  • Ferenci, T.; Stroem, T.; Quayle, J.R.
    Purification and properties of 3-hexulose phosphate synthase and phospho-3-hexuloisomerase from Methylococcus capsulatus (1974), Biochem. J., 144, 477-486.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.43 Ca2+ 49% inhibition at 1 mM Methylococcus capsulatus
4.1.2.43 Cu2+ 24% inhibition at 1 mM Methylococcus capsulatus
4.1.2.43 additional information not inhibited by ATP, ADP, AMP, NADH, D-fructose 6-phosphate, and phosphoenolpyruvate Methylococcus capsulatus
4.1.2.43 Ni2+ 64% inhibition at 1 mM Methylococcus capsulatus
5.3.1.27 Co2+ 1 mM, 35% residual activity Methylococcus capsulatus
5.3.1.27 Cu2+ 1 mM, 8% residual activity Methylococcus capsulatus
5.3.1.27 Mg2+ 5 mM, 30% loss of activity Methylococcus capsulatus
5.3.1.27 additional information at 1 mm sugar phosphate concentration, D-allulose 6-phosphate, D-fructose 6-phosphate, 6-phospho-D-gluconate, D-ribulose 5-phosphate, D-xylulose 5-phosphate, D-erythrose 4-phosphate and glyceraldehyde 3-phosphate do not affect the isomerase activity Methylococcus capsulatus
5.3.1.27 Ni2+ 1 mM, 14% residual activity Methylococcus capsulatus
5.3.1.27 Zn2+ 1 mM, 40% residual activity Methylococcus capsulatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.43 0.075
-
 D-arabino-hex-3-ulose 6-phosphate in 50 mM sodium potassium phosphate buffer, 5 mM MgCl2, at pH 7.0, at 30°C Methylococcus capsulatus
4.1.2.43 0.083
-
 D-ribulose 5-phosphate in 50 mM sodium potassium phosphate buffer, 5 mM MgCl2, at pH 7.0, at 37°C Methylococcus capsulatus
4.1.2.43 0.49
-
 formaldehyde in 50 mM sodium potassium phosphate buffer, 5 mM MgCl2, at pH 7.0, at 37°C Methylococcus capsulatus
5.3.1.27 0.1
-
 D-arabino-3-hexulose 6-phosphate 30°C, pH 7.0 Methylococcus capsulatus
5.3.1.27 1.1
-
 D-fructose 6-phosphate 30°C, pH 7.0 Methylococcus capsulatus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.43 Co2+ 17% as effective as Mg2+ or Mn2+ in promoting synthase activity Methylococcus capsulatus
4.1.2.43 Mg2+ 5 mM, essential for activity and stability, 3-hexulose phosphate synthase is rapidly and apparently irreversibly denatured by storage in the absence of one of Mg2+ Methylococcus capsulatus
4.1.2.43 Mn2+ essential for activity and stability Methylococcus capsulatus
4.1.2.43 Zn2+ 10% as effective as Mg2+ or Mn2+ in promoting synthase activity Methylococcus capsulatus
5.3.1.27 additional information no requirement for divalent cations Methylococcus capsulatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.43 49000
-
 6 * 49000, gel filtration, at pH 4.6 Methylococcus capsulatus
4.1.2.43 310000
-
 gel filtration, under conditions of low pH or low ionic strength Methylococcus capsulatus
5.3.1.27 67000
-
 gel filtration Methylococcus capsulatus

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.43 Methylococcus capsulatus
-
-
-
5.3.1.27 Methylococcus capsulatus
-
 grown on methane
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.43 ammonium sulfate precipitation, DEAE-cellulose column chromatography Methylococcus capsulatus
5.3.1.27
-
 Methylococcus capsulatus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.43 1.71
-
 cell free extract, at 30°C Methylococcus capsulatus
4.1.2.43 69
-
 after 41fold purification, at 30°C Methylococcus capsulatus
5.3.1.27 1560
-
 30°C, pH 7.0 Methylococcus capsulatus

Storage Stability

EC Number Storage Stability Organism
4.1.2.43 -15°C, in the presence of 2.5 mM MgCl2, 6 months, remains stable unless repeatedly frozen and thawed Methylococcus capsulatus
5.3.1.27 -15°C, l0 mM-Tris-HCI, 1 mM-EDTA buffer, pH 7.5, stable for 4 months Methylococcus capsulatus
5.3.1.27 0-4°C, l0 mM-Tris-HCI, 1 mM-EDTA buffer, pH 7.5, stable for 4 months Methylococcus capsulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.43 D-ribulose 5-phosphate + formaldehyde
-
 Methylococcus capsulatus D-arabino-hex-3-ulose 6-phosphate
-
 ?
5.3.1.27 D-arabino-3-hexulose 6-phosphate
-
 Methylococcus capsulatus D-fructose 6-phosphate
-
 r
5.3.1.27 D-fructose 6-phosphate
-
 Methylococcus capsulatus D-arabino-3-hexulose 6-phosphate
-
 r
5.3.1.27 additional information enzyme is specific for substrates D-arabino-3-hexulose 6-phosphate and D-fructose 6-phosphate Methylococcus capsulatus ?
-
 ?
5.3.1.27 additional information no substrate: D-ribulose 5-phosphate, D-xylulose 5-phosphate or D-allulose 6-phosphate Methylococcus capsulatus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.43 homohexamer 6 * 49000, gel filtration, at pH 4.6 Methylococcus capsulatus

Synonyms

EC Number Synonyms Comment Organism
4.1.2.43 3-hexulose phosphate synthase
-
 Methylococcus capsulatus
4.1.2.43 D-arabino-3-hexulose 6-phosphate formaldehyde lyase
-
 Methylococcus capsulatus
4.1.2.43 HPS
-
 Methylococcus capsulatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.2.43 60
-
 3-hexulose phosphate synthase is rapidly inactivated at elevated temperatures, activity is totally lost within 5 min at 60°C Methylococcus capsulatus
5.3.1.27 60
-
 10 min, complete loss of activity Methylococcus capsulatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.43 7
-
-
 Methylococcus capsulatus
5.3.1.27 8.3
-
-
 Methylococcus capsulatus