EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.3.8 | expression in CHO-K1 cells. Secretion of mouse interleukin-2 signal sequence and transmembrane domain truncated human GD3-synthase cDNA amino-acid residues 49-356 | Homo sapiens |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.4.3.8 | secondary structure alignments between Campylobacter jejunii sialyltransferase CstII and human GD3-synthase. In the human enzyme, the side chain on residue N188 has a strong hydrogen bond with the carboxyl group on the sialic acid group of the donor substrate. Residue P189 has no interaction with the donor. The distance between S190 and the donor substrate is 4.4 A, this residue might weakly interact with the donor. R272 is 8.8 A away from the donor, suggesting that this residue has no function on donor substrate binding | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.3.8 | N188D | mutation in a conserved residue identified by structure alignments between Campylobacter jejunii sialyltransferase CstII and human GD3-synthase, 6fold decrease in ratio Km to Vmax | Homo sapiens |
2.4.3.8 | R272A | mutation in a conserved residue identified by structure alignments between Campylobacter jejunii sialyltransferase CstII and human GD3-synthase, 4fold decrease in ratio Km to Vmax | Homo sapiens |
2.4.3.8 | S190A | mutation in a conserved residue identified by structure alignments between Campylobacter jejunii sialyltransferase CstII and human GD3-synthase, 4fold decrease in ratio Km to Vmax | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.3.8 | 0.081 | - |
CMP-N-acetylneuraminate | mutant R272A, pH 6.5, 37°C | Homo sapiens | |
2.4.3.8 | 0.083 | - |
ganglioside GM3 | wild-type, pH 6.5, 37°C | Homo sapiens | |
2.4.3.8 | 0.087 | - |
ganglioside GM3 | mutant N188D, pH 6.5, 37°C | Homo sapiens | |
2.4.3.8 | 0.088 | - |
CMP-N-acetylneuraminate | wild-type, pH 6.5, 37°C | Homo sapiens | |
2.4.3.8 | 0.11 | - |
CMP-N-acetylneuraminate | mutant S190A, pH 6.5, 37°C | Homo sapiens | |
2.4.3.8 | 0.184 | - |
ganglioside GM3 | mutant S190A, pH 6.5, 37°C | Homo sapiens | |
2.4.3.8 | 0.22 | - |
CMP-N-acetylneuraminate | mutant N188D, pH 6.5, 37°C | Homo sapiens | |
2.4.3.8 | 0.979 | - |
ganglioside GM3 | mutant R272A, pH 6.5, 37°C | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.3.8 | Homo sapiens | Q92185 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.3.8 | CMP-N-acetylneuraminate + ganglioside GM3 | - |
Homo sapiens | CMP + ? | - |
? |