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Literature summary extracted from

  • Casanova, J.C.; Kuhn, J.; Kleesiek, K.; Goetting, C.
    Heterologous expression and biochemical characterization of soluble human xylosyltransferase II (2008), Biochem. Biophys. Res. Commun., 365, 678-684.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.2.26 EDTA 84% activation at 25 mM Homo sapiens
2.4.2.26 histone protein activity gains 1.5fold when histone protein is added at a concentration of 0.1 mg/ml Homo sapiens
2.4.2.26 protamine adding protamine to a final concentration of 0.1 mg/ml stimulates XT-II activity at least 2fold Homo sapiens
2.4.2.26 Sodium citrate 72% activation at 25 mM Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.26 expressed in Pichia pastoris strain X-33 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.26 beta-mercaptoethanol XT-II activity is completely abolished at a concentration 1% (v/v) beta-mercaptoethanol Homo sapiens
2.4.2.26 CDP 23% residual activity at 10 mg/ml Homo sapiens
2.4.2.26 CMP 45% residual activity at 10 mg/ml Homo sapiens
2.4.2.26 Co2+ Co2+ reduces activity by more than 60% at 5 mM Homo sapiens
2.4.2.26 CTP 8% residual activity at 10 mg/ml Homo sapiens
2.4.2.26 Cu2+ complete inhibition at 5 mM Homo sapiens
2.4.2.26 dithiothreitol XT-II activity is completely abolished at a concentration of 1 mM dithiothreitol Homo sapiens
2.4.2.26 additional information lysozyme does not change the activity of XT-II at any concentration Homo sapiens
2.4.2.26 Ni2+ complete inhibition at 5 mM Homo sapiens
2.4.2.26 protamine adding protamine to a final concentration of 10 mg/ml decreases XT-II activity 4fold Homo sapiens
2.4.2.26 UDP 5% residual activity at 10 mg/ml Homo sapiens
2.4.2.26 UMP 25% residual activity at 10 mg/ml Homo sapiens
2.4.2.26 UTP 4% residual activity at 10 mg/ml Homo sapiens
2.4.2.26 Zn2+ complete inhibition at 5 mM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.26 0.0019
-
 Bio-QEEEGSGGGQKK-F
-
 Homo sapiens
2.4.2.26 0.0108
-
 bikunin
-
 Homo sapiens
2.4.2.26 0.0191
-
 fibroblast growth factor 2 fragment 1-24
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.26 Mg2+ necessity for Mg2+ or Mn2+ ions for enzymatic activity Homo sapiens
2.4.2.26 Mn2+ necessity for Mg2+ or Mn2+ ions for enzymatic activity Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.26 110000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.26 Homo sapiens Q9H1B5
-
-

Storage Stability

EC Number Storage Stability Organism
2.4.2.26 -80°C, -20°C or 4°C, 3-4 weeks, no loss of activity Homo sapiens
2.4.2.26 25°C, 2 weeks, no loss of activity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.26 additional information TENEGSGLTNIK, SENEGSGMAEQK, orcokinin (NFDEIDRSGFGFN), melittin (GIGAVLKVLTTGLPALISWIKRKRQQ), fibrinopeptide A (ADSGEGDFLAEGGGVR), IgE C4 domain (KTKGSGFFVF), C-type natriuretic peptide (GLSKGCFGLKLDRIGSMSGLGC), and calcitonin (VLGKLSQELHKLQTYPRTNTGSGTP) are no substrates for XT-II Homo sapiens ?
-
 ?
2.4.2.26 UDP-D-xylose + bikunin
-
 Homo sapiens UDP + D-xylosyl-bikunin
-
 ?
2.4.2.26 UDP-D-xylose + biotin-NH-QEEEGSGGGQKK(5-fluorescein)-CONH2 the bikunin-homologous peptide Bio-QEEEGSGGGQKK-F is best acceptor substrate for XT-II, 100% activity with UDP-D-xylose Homo sapiens UDP + biotin-NH-QEEEG-(D-xylosyl)SGGGQKK(5-fluorescein)-CONH2
-
 ?
2.4.2.26 UDP-D-xylose + fibroblast growth factor 2 fragment 1-24
-
 Homo sapiens ?
-
 ?
2.4.2.26 UDP-D-xylose + silk fibroin
-
 Homo sapiens UDP + silk fibroin with xylosylserine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.26 XT-II
-
 Homo sapiens
2.4.2.26 xylosyltransferase II isoform of xylosyltransferase I Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.26 30
-
-
 Homo sapiens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.2.26 4 45
-
 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.26 37
-
 reaching a temperature of 37°C, XT-II has 66% of the activity compared with 30°C Homo sapiens