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Literature summary extracted from

  • Pramfalk, C.; Angelin, B.; Eriksson, M.; Parini, P.
    Cholesterol regulates ACAT2 gene expression and enzyme activity in human hepatoma cells (2007), Biochem. Biophys. Res. Commun., 364, 402-409.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.26 cholesterol ACAT2 is transcriptionally regulated by cholesterol Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.26 membrane
-
 Homo sapiens 16020
-
2.3.1.26 rough endoplasmic reticulum
-
 Homo sapiens 5791
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.26 acyl-CoA + cholesterol Homo sapiens
-
 CoA + cholesterol ester
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.26 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.26 microsomes are prepared Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.26 enterocyte
-
 Homo sapiens
-
2.3.1.26 Hep-G2 cell
-
 Homo sapiens
-
2.3.1.26 hepatocyte
-
 Homo sapiens
-
2.3.1.26 hepatoma cell
-
 Homo sapiens
-
2.3.1.26 Huh-7 cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.26 0.0127
-
 ACAT2 activity in HuH-7 cells, control Homo sapiens
2.3.1.26 0.0192
-
 ACAT2 activity in HuH-7 cells, 30 microM HDL cholesterol Homo sapiens
2.3.1.26 0.0347
-
 ACAT2 activity in HuH-7 cells, 0.5 mM LDL cholesterol Homo sapiens
2.3.1.26 0.039
-
 ACAT2 activity in HuH-7 cells, control Homo sapiens
2.3.1.26 0.0558
-
 ACAT1 activity in HuH-7 cells, control Homo sapiens
2.3.1.26 0.0701
-
 ACAT1 activity in HuH-7 cells, 0.5 mM LDL cholesterol Homo sapiens
2.3.1.26 0.086
-
 ACAT1 activity in HuH-7 cells, control Homo sapiens
2.3.1.26 0.0946
-
 ACAT2 activity in HuH-7 cells, 0.1 mM free cholesterol Homo sapiens
2.3.1.26 0.1102
-
 ACAT1 activity in HuH-7 cells, 0.1 mM free cholesterol Homo sapiens
2.3.1.26 0.1861
-
 ACAT1 activity in HuH-7 cells, 30 microM HDL cholesterol Homo sapiens
2.3.1.26 2210
-
 ACAT2 activity in Hep-G2 cells, control Homo sapiens
2.3.1.26 3220
-
 ACAT2 activity in Hep-G2 cells, control Homo sapiens
2.3.1.26 4650
-
 ACAT1 activity in Hep-G2 cells, 1.5 mM LDL cholesterol Homo sapiens
2.3.1.26 4730
-
 ACAT1 activity in Hep-G2 cells, 1 mM HDL cholesterol Homo sapiens
2.3.1.26 4980
-
 ACAT1 activity in Hep-G2 cells, control Homo sapiens
2.3.1.26 5030
-
 ACAT2 activity in Hep-G2 cells, 1.5 mM LDL cholesterol Homo sapiens
2.3.1.26 5750
-
 ACAT1 activity in Hep-G2 cells, control Homo sapiens
2.3.1.26 5810
-
 ACAT1 activity in Hep-G2 cells, 0.9 mM free cholesterol Homo sapiens
2.3.1.26 6230
-
 ACAT2 activity in Hep-G2 cells, 1 mM HDL cholesterol Homo sapiens
2.3.1.26 9560
-
 ACAT2 activity in Hep-G2 cells, 0.9 mM free cholesterol Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.26 acyl-CoA + cholesterol
-
 Homo sapiens CoA + cholesterol ester
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.26 ACAT1
-
 Homo sapiens
2.3.1.26 ACAT2
-
 Homo sapiens
2.3.1.26 acyl-coenzyme A:cholesterol acyltransferase
-
 Homo sapiens
2.3.1.26 Soat1
-
 Homo sapiens
2.3.1.26 Soat2
-
 Homo sapiens
2.3.1.26 steroyl O-acyltransferase 1
-
 Homo sapiens
2.3.1.26 steroyl O-acyltransferase 2
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.26 37
-
 activity assay Homo sapiens