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Literature summary extracted from

  • Grill, S.; Yamaguchi, H.; Wagner, H.; Zwahlen, L.; Kusch, U.; Mack, M.
    Identification and characterization of two Streptomyces davawensis riboflavin biosynthesis gene clusters (2007), Arch. Microbiol., 188, 377-387.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.193 gene ribG, ribG forms an expression unit with ribY, a monofunctional GTP cyclohydrolase II, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain BL21(DE3) Streptomyces davaonensis
2.5.1.9 expression in Escherichia coli Streptomyces davaonensis

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.193 additional information a ribG mutant can be rescued by expression of ribB, overview Streptomyces davaonensis
2.5.1.9 additional information overexpression in Escherichia coli can suppress the lack of pyrimidine deaminase/reductase in a riboflavin-deficient ribD- strain Streptomyces davaonensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.193 22000
-
 x * 22000, recombinant enzyme, SDS-PAGE Streptomyces davaonensis
2.5.1.9 22000
-
 x * 22000, SDS-PAGE Streptomyces davaonensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.193 5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADPH + H+ Streptomyces davaonensis
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADP+
-
 ?
1.1.1.193 5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADPH + H+ Streptomyces davaonensis 768
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADP+
-
 ?
1.1.1.193 additional information Streptomyces davaonensis the enzyme is involved in riboflavin biosynthesis, overview ?
-
 ?
1.1.1.193 additional information Streptomyces davaonensis 768 the enzyme is involved in riboflavin biosynthesis, overview ?
-
 ?
2.5.1.78 5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate Streptomyces davaonensis the enzyme is part of the riboflavin biosynthesis gene cluster 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.193 Streptomyces davaonensis Q2VCF5 bifunctional enzyme; gene ribG
-
1.1.1.193 Streptomyces davaonensis 768 Q2VCF5 bifunctional enzyme; gene ribG
-
2.5.1.9 Streptomyces davaonensis Q2VCE7
-
-
2.5.1.78 Streptomyces davaonensis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.193 5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADPH + H+
-
 Streptomyces davaonensis 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADP+
-
 ?
1.1.1.193 5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADPH + H+
-
 Streptomyces davaonensis 768 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate + NADP+
-
 ?
1.1.1.193 additional information the enzyme is involved in riboflavin biosynthesis, overview Streptomyces davaonensis ?
-
 ?
1.1.1.193 additional information the enzyme is involved in riboflavin biosynthesis, overview Streptomyces davaonensis 768 ?
-
 ?
2.5.1.78 5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate penultimate step of riboflavin biosynthesis Streptomyces davaonensis 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O
-
 ?
2.5.1.78 5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate the enzyme is part of the riboflavin biosynthesis gene cluster Streptomyces davaonensis 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.193 ? x * 22000, recombinant enzyme, SDS-PAGE Streptomyces davaonensis
2.5.1.9 ? x * 22000, SDS-PAGE Streptomyces davaonensis
2.5.1.9 More N-terminal sequence is MFTGIVEELGEITAV Streptomyces davaonensis

Synonyms

EC Number Synonyms Comment Organism
2.5.1.78 RibH
-
 Streptomyces davaonensis