Any feedback?
Literature summary extracted from
- Degradation of beta-hexachlorocyclohexane by haloalkane dehalogenase LinB from gamma-hexachlorocyclohexane-utilizing bacterium Sphingobium sp. MI1205 (2007), Arch. Microbiol., 188, 313-325.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.8.1.5 | using the plasmids pKS13, pUC18, pUC19 and pAQN, the plasmid pAQN is used for epression of the protein in Escherichia coli BL21 cells | Sphingobium indicum |
| 3.8.1.5 | using the plasmids pKS13, pUC18, pUC19 and pAQNM, the plasmid pAQNM is used for expression of the protein in Escherichia coli BL21 cells | Sphingobium sp. |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.8.1.5 | A247H | mutant, constructed for quantitative evaluation of the differences between LinB of Sphingobium japonicum and LinB of Sphingobium sp. | Sphingobium indicum |
| 3.8.1.5 | H247A | mutant, constructed for quantitative evaluation of the differences between LinB of Sphingobium japonicum and LinB of Sphingobium sp. | Sphingobium sp. |
| 3.8.1.5 | I134A | mutant, constructed for quantitative evaluation of the differences between LinB of Sphingobium japonicum and LinB of Sphingobium sp. | Sphingobium indicum |
| 3.8.1.5 | I134V/A247H | mutant, constructed for quantitative evaluation of the differences between LinB of Sphingobium japonicum and LinB of Sphingobium sp. | Sphingobium indicum |
| 3.8.1.5 | V134I | mutant, constructed for quantitative evaluation of the differences between LinB of Sphingobium japonicum and LinB of Sphingobium sp. | Sphingobium sp. |
| 3.8.1.5 | V134I/H247A | mutant, constructed for quantitative evaluation of the differences between LinB of Sphingobium japonicum and LinB of Sphingobium sp. | Sphingobium sp. |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.8.1.5 | 2,3,4,5,6-pentachlorocyclohexanol + H2O | Sphingobium sp. | - |
2,3,5,6-tetrachlorocyclohexane-1,4-diol + chloride | - |
? |  |
| 3.8.1.5 | 2,3,4,5,6-pentachlorocyclohexanol + H2O | Sphingobium sp. MI1205 | - |
2,3,5,6-tetrachlorocyclohexane-1,4-diol + chloride | - |
? | |
| 3.8.1.5 | beta-hexachlorocyclohexane + H2O | Sphingobium indicum | - |
2,3,4,5,6-pentachlorocyclohexanol + chloride | - |
? | |
| 3.8.1.5 | beta-hexachlorocyclohexane + H2O | Sphingobium sp. | two-step conversion of beta-HCH to TCDL | 2,3,4,5,6-pentachlorocyclohexanol + chloride | - |
? | |
| 3.8.1.5 | beta-hexachlorocyclohexane + H2O | Sphingobium sp. MI1205 | two-step conversion of beta-HCH to TCDL | 2,3,4,5,6-pentachlorocyclohexanol + chloride | - |
? | |
| 3.8.1.5 | beta-hexachlorocyclohexane + H2O | Sphingobium indicum UT26 | - |
2,3,4,5,6-pentachlorocyclohexanol + chloride | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.8.1.5 | Sphingobium indicum | A8CFB7 | - |
- |
| 3.8.1.5 | Sphingobium indicum UT26 | A8CFB7 | - |
- |
| 3.8.1.5 | Sphingobium sp. | A4PEU6 | - |
- |
| 3.8.1.5 | Sphingobium sp. MI1205 | A4PEU6 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.8.1.5 | using TALON metal affinity resin | Sphingobium indicum |
| 3.8.1.5 | using TALON metal affinity resin | Sphingobium sp. |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.8.1.5 | additional information | - |
specific constant of LinB, mutant A247H, step HCH to PCHL, 0.0271 mM-1 s-1 | Sphingobium indicum |
| 3.8.1.5 | additional information | - |
specific constant of LinB, mutant A247H, step PCHL to TCDL, not determined | Sphingobium indicum |
| 3.8.1.5 | additional information | - |
specific constant of LinB, mutant H247A, step HCH to PCHL, 0.210 mM-1 s-1 | Sphingobium sp. |
| 3.8.1.5 | additional information | - |
specific constant of LinB, mutant H247A, step PCHL to TCDL, 0.240 mM-1 s-1 | Sphingobium sp. |
| 3.8.1.5 | additional information | - |
specific constant of LinB, mutant I134V, step HCH to PCHL, 0.0969 mM-1 s-1 | Sphingobium indicum |
| 3.8.1.5 | additional information | - |
specific constant of LinB, mutant I134V, step PCHL to TCDL, 0.0099 mM-1 s-1 | Sphingobium indicum |
| 3.8.1.5 | additional information | - |
specific constant of LinB, mutant I134V/A247H, step HCH to PCHL, 0.0243 mM-1 s-1 | Sphingobium indicum |
| 3.8.1.5 | additional information | - |
specific constant of LinB, mutant I134V/A247H, step PCHL to TCDL, 0.0183 mM-1 s-1 | Sphingobium indicum |
| 3.8.1.5 | additional information | - |
specific constant of LinB, mutant V134I, step HCH to PCHL, 0.124 mM-1 s-1 | Sphingobium sp. |
| 3.8.1.5 | additional information | - |
specific constant of LinB, mutant V134I, step PCHL to TCDL, 0.080 mM-1 s-1 | Sphingobium sp. |
| 3.8.1.5 | additional information | - |
specific constant of LinB, mutant V134I/H247A, step HCH to PCHL, 0.104 mM-1 s-1 | Sphingobium sp. |
| 3.8.1.5 | additional information | - |
specific constant of LinB, mutant V134I/H247A, step PCHL to TCDL, 0.027 mM-1 s-1 | Sphingobium sp. |
| 3.8.1.5 | additional information | - |
specific constant of LinB, wild-type, step HCH to PCHL, 0.0271 mM-1 s-1 | Sphingobium indicum |
| 3.8.1.5 | additional information | - |
specific constant of LinB, wild-type, step HCH to PCHL, 0.205 mM-1 s-1 | Sphingobium sp. |
| 3.8.1.5 | additional information | - |
specific constant of LinB, wild-type, step PCHL to TCDL, 0.0036 mM-1 s-1 | Sphingobium indicum |
| 3.8.1.5 | additional information | - |
specific constant of LinB, wild-type, step PCHL to TCDL, 0.716 mM-1 s-1 | Sphingobium sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.8.1.5 | 2,3,4,5,6-pentachlorocyclohexanol + H2O | - |
Sphingobium sp. | 2,3,5,6-tetrachlorocyclohexane-1,4-diol + chloride | - |
? | |
| 3.8.1.5 | 2,3,4,5,6-pentachlorocyclohexanol + H2O | - |
Sphingobium sp. MI1205 | 2,3,5,6-tetrachlorocyclohexane-1,4-diol + chloride | - |
? | |
| 3.8.1.5 | beta-hexachlorocyclohexane + H2O | - |
Sphingobium indicum | 2,3,4,5,6-pentachlorocyclohexanol + chloride | - |
? | |
| 3.8.1.5 | beta-hexachlorocyclohexane + H2O | two-step conversion of beta-HCH to TCDL | Sphingobium sp. | 2,3,4,5,6-pentachlorocyclohexanol + chloride | - |
? | |
| 3.8.1.5 | beta-hexachlorocyclohexane + H2O | two-step conversion of beta-HCH to TCDL | Sphingobium sp. MI1205 | 2,3,4,5,6-pentachlorocyclohexanol + chloride | - |
? | |
| 3.8.1.5 | beta-hexachlorocyclohexane + H2O | - |
Sphingobium indicum UT26 | 2,3,4,5,6-pentachlorocyclohexanol + chloride | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.8.1.5 | haloalkane dehalogenase | - |
Sphingobium indicum |
| 3.8.1.5 | haloalkane dehalogenase | - |
Sphingobium sp. |
| 3.8.1.5 | LinB | - |
Sphingobium indicum |
| 3.8.1.5 | LinB | - |
Sphingobium sp. |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.8.1.5 | 30 | - |
enzymatic assay | Sphingobium indicum |
| 3.8.1.5 | 30 | - |
enzymatic assay | Sphingobium sp. |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.8.1.5 | 7.5 | - |
enzymatic assay | Sphingobium indicum |
| 3.8.1.5 | 7.5 | - |
enzymatic assay | Sphingobium sp. |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
