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Literature summary extracted from

  • Torres-Rivera, A.; Landa, A.
    Cooperative kinetics of the recombinant glutathione transferase of Taenia solium and characterization of the enzyme (2008), Arch. Biochem. Biophys., 477, 372-378.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.18 expressed in Escherichia coli strain JM105 Taenia solium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 0.2
-
 glutathione recombinant enzyme, at 25°C Taenia solium
2.5.1.18 1.06
-
 1-chloro-2,4-dinitrobenzene recombinant enzyme, at 25°C Taenia solium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.18 cytosol
-
 Taenia solium 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.18 26000
-
 around 26000 Da, SDS-PAGE Taenia solium
2.5.1.18 26500
-
 gel filtration Taenia solium

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Taenia solium Q3ZJN3 fragment
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18 Sepharose-glutathione column chromatography Taenia solium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.18 0.22
-
 using 1,2-dichloro-4-nitrobenzene as a substrate Taenia solium
2.5.1.18 0.4
-
 using trans-2-nonenal as a substrate Taenia solium
2.5.1.18 0.53
-
 using hexa 2,4-dienal as a substrate Taenia solium
2.5.1.18 2.48
-
 using cumene hydroperoxide as a substrate Taenia solium
2.5.1.18 3.51
-
 using ethacrynic acid as a substrate Taenia solium
2.5.1.18 51.5
-
 using 1-chloro-2,4-dinitrobenzene as a substrate Taenia solium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 glutathione + 1,2-dichloro-4-nitrobenzene
-
 Taenia solium ?
-
 ?
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene preferred substrate Taenia solium S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?
2.5.1.18 glutathione + cumene hydroperoxide
-
 Taenia solium ?
-
 ?
2.5.1.18 glutathione + ethacrynic acid
-
 Taenia solium ?
-
 ?
2.5.1.18 glutathione + hexa 2,4-dienal
-
 Taenia solium ?
-
 ?
2.5.1.18 glutathione + trans-2-nonenal
-
 Taenia solium ?
-
 ?
2.5.1.18 additional information no activity with 1,2-epoxy-3-(p-nitrophenoxy)-propane and bromosulfophthalein Taenia solium ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 26GST 26.5 kDa isozyme Taenia solium
2.5.1.18 glutathione S-transferase
-
 Taenia solium
2.5.1.18 GST
-
 Taenia solium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.18 22.2
-
 1-chloro-2,4-dinitrobenzene recombinant enzyme, at 25°C Taenia solium