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Literature summary extracted from
- A pyrroloquinoline quinine-dependent membrane-bound d-sorbitol dehydrogenase from Gluconobacter oxydans exhibits an ordered Bi Bi reaction mechanism (2008), Arch. Biochem. Biophys., 477, 206-210.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.21 | D-sorbose | non-competitive inhibition, sorbitol or phenazine methosulfate as substrates | Gluconobacter oxydans |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.99.21 | 0.0257 | - |
ubiquinone-2 | - |
Gluconobacter oxydans | |
| 1.1.99.21 | 0.0314 | - |
phenazine methosulfate | - |
Gluconobacter oxydans | |
| 1.1.99.21 | 16.8 | - |
D-sorbitol | ubiquinone-2 as electron acceptor | Gluconobacter oxydans | |
| 1.1.99.21 | 23.2 | - |
D-sorbitol | phenazine methosulfate as electron acceptor | Gluconobacter oxydans | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.99.21 | Gluconobacter oxydans | - |
IFO 3255 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.99.21 | by CM-cellulose and sephacryl HR 400 column chromatography | Gluconobacter oxydans |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.99.21 | stable at 80°C for more than two months in the presence of 0.1% Tween-20 | Gluconobacter oxydans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.99.21 | D-sorbitol + phenazine methosulfate | the catalytic reaction follows an ordered Bi Bi mechanism, the native mSLDH bears two different substrate-binding sites, one for ubiquinone using as electron acceptor and the other for D-sorbitol, in addition to PQQ-binding and Mg2+-binding sites in the catalytic center | Gluconobacter oxydans | ? | - |
? | |
| 1.1.99.21 | D-sorbitol + ubiquinone-2 | - |
Gluconobacter oxydans | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.99.21 | D-sorbitol dehydrogenase | membrane-bound pyrroloquinoline quinine dependent, mSLDH | Gluconobacter oxydans |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.21 | additional information | pyrroloquinoline quinine | Gluconobacter oxydans |
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Release 2023.1 (January 2023)
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