EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.16.1 | E330H | site-directed mutagenesis of a metal ligand binding residue, the mutant enzyme shows over 80% reduced activity compared to the wild-type enzyme | Rattus norvegicus |
1.14.16.1 | E330Q | site-directed mutagenesis of a metal ligand binding residue, the mutant enzyme shows over 80% reduced activity compared to the wild-type enzyme | Rattus norvegicus |
1.14.16.1 | H285E | site-directed mutagenesis of a metal ligand binding residue, the mutant enzyme shows over 80% reduced activity compared to the wild-type enzyme | Rattus norvegicus |
1.14.16.1 | H285Q | site-directed mutagenesis of a metal ligand binding residue, the mutant enzyme shows 80% reduced activity compared to the wild-type enzyme | Rattus norvegicus |
1.14.16.1 | H290E | site-directed mutagenesis of a metal ligand binding residue, the mutant enzyme shows over 80% reduced activity compared to the wild-type enzyme | Rattus norvegicus |
1.14.16.1 | H290Q | site-directed mutagenesis of a metal ligand binding residue, the mutant enzyme shows over 80% reduced activity compared to the wild-type enzyme | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.16.1 | additional information | - |
additional information | binding constants for Fe2+ of wild-type and mutant enzymes, overview | Rattus norvegicus | |
1.14.16.1 | 0.43 | - |
L-Phe | pH 7.0, 25°C, wild-type enzyme | Rattus norvegicus | |
1.14.16.1 | 0.43 | - |
6-methyltetrahydrobiopterin | pH 7.0, 25°C, wild-type enzyme | Rattus norvegicus | |
1.14.16.1 | 0.43 | - |
6-methyltetrahydrobiopterin | pH 7.0, 25°C, mutant E330H | Rattus norvegicus | |
1.14.16.1 | 0.43 | - |
6-methyltetrahydrobiopterin | pH 7.0, 25°C, mutant H285E | Rattus norvegicus | |
1.14.16.1 | 1.2 | - |
L-Phe | pH 7.0, 25°C, mutant H290Q | Rattus norvegicus | |
1.14.16.1 | 1.2 | - |
6-methyltetrahydrobiopterin | pH 7.0, 25°C, mutant H290Q | Rattus norvegicus | |
1.14.16.1 | 1.6 | - |
L-Phe | pH 7.0, 25°C, mutant E330Q | Rattus norvegicus | |
1.14.16.1 | 1.6 | - |
6-methyltetrahydrobiopterin | pH 7.0, 25°C, mutant E330Q | Rattus norvegicus | |
1.14.16.1 | 3.4 | - |
L-Phe | pH 7.0, 25°C, mutant H290E | Rattus norvegicus | |
1.14.16.1 | 3.4 | - |
6-methyltetrahydrobiopterin | pH 7.0, 25°C, mutant H290E | Rattus norvegicus | |
1.14.16.1 | 6.9 | - |
L-Phe | pH 7.0, 25°C, mutant H285Q | Rattus norvegicus | |
1.14.16.1 | 6.9 | - |
6-methyltetrahydrobiopterin | pH 7.0, 25°C, mutant H285Q | Rattus norvegicus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.16.1 | Fe2+ | nonheme iron is bound on one face by residues His285, His290, and Glu330 forming a a 2-His-1-carboxylate facial triad, the three ligands differ in their sensitivity to mutagenesis, structure, overview | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.16.1 | L-phenylalanine + tetrahydrobiopterin + O2 | Rattus norvegicus | - |
L-tyrosine + 4a-hydroxytetrahydrobiopterin | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.16.1 | Rattus norvegicus | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.16.1 | L-phenylalanine + 6-methyltetrahydrobiopterin + O2 | - |
Rattus norvegicus | L-tyrosine + 6-methyl-4a-hydroxytetrahydrobiopterin | - |
? | |
1.14.16.1 | L-phenylalanine + tetrahydrobiopterin + O2 | - |
Rattus norvegicus | L-tyrosine + 4a-hydroxytetrahydrobiopterin | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.16.1 | PAH | - |
Rattus norvegicus |
1.14.16.1 | phenylalanine hydroxylase | - |
Rattus norvegicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.16.1 | 25 | - |
assay at | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.16.1 | 7 | - |
assay at | Rattus norvegicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.16.1 | 6-methyltetrahydrobiopterin | - |
Rattus norvegicus | |
1.14.16.1 | tetrahydrobiopterin | - |
Rattus norvegicus |