EC Number | Cloned (Comment) | Organism |
---|---|---|
1.15.1.1 | gene KmSod1, DNA and amino acid sequence determination and analysis, sequence comparisons, overexpression of Cu/Zn-SOD under the control of the KlADH4 promoter in strain L3 from a multicopy plasmid, subcloning in Escherichia coli strain DH5alpha | Kluyveromyces marxianus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.15.1.1 | additional information | overexpression of recombinant Cu/Zn-SOD1 in strain L3 does not significantly increase the SOD specific activity, and in some cases, a net reduction of the enzymatic activity occurs, cell phenotypes, overview | Kluyveromyces marxianus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.15.1.1 | Cu2+ | isozyme Cu/Zn-SOD, the highly conserved histidine residues H47, H49, H64, H72, H81, and H121 are involved in the interaction with the metallic cofactors, which are essential for activity and folding in all the Sod1 enzymes, D84 is also involve in Cu2+ binding | Kluyveromyces marxianus | |
1.15.1.1 | Zn2+ | isozyme Cu/Zn-SOD, the highly conserved histidine residues H47, H49, H64, H72, H81, and H121 are involved in the interaction with the metallic cofactors, which are essential for activity and folding in all the Sod1 enzymes | Kluyveromyces marxianus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.15.1.1 | 15882 | - |
x * 15882, Cu/Zn-SOD sequence calculation | Kluyveromyces marxianus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.15.1.1 | additional information | Kluyveromyces marxianus | SOD activity is one major defense line against oxidative stress for all of the aerobic organisms | ? | - |
? | |
1.15.1.1 | additional information | Kluyveromyces marxianus L3 | SOD activity is one major defense line against oxidative stress for all of the aerobic organisms | ? | - |
? | |
1.15.1.1 | O2.- + H+ | Kluyveromyces marxianus | - |
O2 + H2O2 | - |
? | |
1.15.1.1 | O2.- + H+ | Kluyveromyces marxianus L3 | - |
O2 + H2O2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.15.1.1 | Kluyveromyces marxianus | B0B552 | SOD1; gene KmSod1 | - |
1.15.1.1 | Kluyveromyces marxianus L3 | B0B552 | SOD1; gene KmSod1 | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.15.1.1 | additional information | - |
Cu/Zn-SOD activity in overexpressing cells from different culture conditions, overview | Kluyveromyces marxianus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.15.1.1 | additional information | SOD activity is one major defense line against oxidative stress for all of the aerobic organisms | Kluyveromyces marxianus | ? | - |
? | |
1.15.1.1 | additional information | SOD activity is one major defense line against oxidative stress for all of the aerobic organisms | Kluyveromyces marxianus L3 | ? | - |
? | |
1.15.1.1 | O2.- + H+ | - |
Kluyveromyces marxianus | O2 + H2O2 | - |
? | |
1.15.1.1 | O2.- + H+ | - |
Kluyveromyces marxianus L3 | O2 + H2O2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.15.1.1 | ? | x * 15882, Cu/Zn-SOD sequence calculation | Kluyveromyces marxianus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.15.1.1 | Cu/Zn superoxide dismutase | - |
Kluyveromyces marxianus |
1.15.1.1 | Cu/Zn-SOD | - |
Kluyveromyces marxianus |
1.15.1.1 | KmSod1p | - |
Kluyveromyces marxianus |
1.15.1.1 | SOD | - |
Kluyveromyces marxianus |
1.15.1.1 | SOD1 | - |
Kluyveromyces marxianus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.15.1.1 | 25 | - |
assay at | Kluyveromyces marxianus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.15.1.1 | 7.8 | - |
assay at | Kluyveromyces marxianus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.15.1.1 | Kluyveromyces marxianus | Cu/Zn-SOD sequence calculation | - |
6.39 |