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Literature summary extracted from

  • Lee, W.H.; Park, J.B.; Park, K.; Kim, M.D.; Seo, J.H.
    Enhanced production of epsilon-caprolactone by overexpression of NADPH-regenerating glucose 6-phosphate dehydrogenase in recombinant Escherichia coli harboring cyclohexanone monooxygenase gene (2007), Appl. Microbiol. Biotechnol., 76, 329-338.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.22 expression in Escherichia coli, optimization of CHMO expression conditions, high concentrations of cyclohexanone cause the formation of CHMO as inclusion bodies during the reaction period, co-expression with the NADPH-regenerating enzyme glucose 6-phosphate dehydrogenase, overview Acinetobacter calcoaceticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.22 cyclohexanone substrate inhibition Acinetobacter calcoaceticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.22 cyclohexanone + NADPH + H+ + O2 Acinetobacter calcoaceticus
-
 epsilon-caprolactone + NADP+ + H2O
-
 ?
1.14.13.22 cyclohexanone + NADPH + H+ + O2 Acinetobacter calcoaceticus NCIMB 9871
-
 epsilon-caprolactone + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.22 Acinetobacter calcoaceticus
-
-
-
1.14.13.22 Acinetobacter calcoaceticus NCIMB 9871
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.22 cyclohexanone + NADPH + H+ + O2
-
 Acinetobacter calcoaceticus epsilon-caprolactone + NADP+ + H2O
-
 ?
1.14.13.22 cyclohexanone + NADPH + H+ + O2
-
 Acinetobacter calcoaceticus NCIMB 9871 epsilon-caprolactone + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.22 CHMO
-
 Acinetobacter calcoaceticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.22 30
-
 assay at Acinetobacter calcoaceticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.22 7
-
 assay at Acinetobacter calcoaceticus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.22 NADPH
-
 Acinetobacter calcoaceticus