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Literature summary extracted from

  • Ilmen, M.; Koivuranta, K.; Ruohonen, L.; Suominen, P.; Penttila, M.
    Efficient production of L-lactic acid from xylose by Pichia stipitis (2007), Appl. Environ. Microbiol., 73, 117-123.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.1.1.27 synthesis development of yeast-based bioprocesses to produce lactate from lignocellulosic raw material Lactobacillus helveticus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.27 expression in Pichia stipidis strain CBS6054, subcloning in Escherichia coli strain DH5alpha Lactobacillus helveticus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.27 additional information construction of a Pichia stipitis strain CBS6054, that expresses the LDH from Lactobacillus helveticus under the control of the Pichia stipitis fermentative ADH1 promoter, the mutant yeast uses xylose, glucose, or a mixture of the two sugars as the carbon source for lactate production, LDH competes efficiently with the ethanol pathway for pyruvate, overview Lactobacillus helveticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.27 (S)-lactate + NAD+ Lactobacillus helveticus
-
 pyruvate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.27 Lactobacillus helveticus
-
 gene ldhL
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.27 (S)-lactate + NAD+
-
 Lactobacillus helveticus pyruvate + NADH + H+
-
 r

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.27 NAD+
-
 Lactobacillus helveticus
1.1.1.27 NADH
-
 Lactobacillus helveticus