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Literature summary extracted from

  • Zarnt, G.; Schraeder, T.; Andreesen, J.R.
    Degradation of tetrahydrofurfuryl alcohol by Ralstonia eutropha is initiated by an inducible pyrroloquinoline quinone-dependent alcohol dehydrogenase (1997), Appl. Environ. Microbiol., 63, 4891-4898.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.5.2 additional information tetrahydrofurfuryl alcohol induces the enzyme, while the ferricyanide-dependent ADH activity is induced during growth on different alcohols except ethanol Cupriavidus necator

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.5.2 EDTA
-
 Cupriavidus necator

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.5.2 Ca2+ required for activity, activates Cupriavidus necator

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.5.2 73600
-
 1 * 73600, SDS-PAGE Cupriavidus necator
1.2.5.2 79500
-
 gel filtration Cupriavidus necator

Organism

EC Number Organism UniProt Comment Textmining
1.2.5.2 Cupriavidus necator
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.5.2 native enzyme 211fold to homogeneity Cupriavidus necator

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.5.2 culture condition:tetrahydrofurfuryl alcohol-grown cell
-
 Cupriavidus necator
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.5.2 39.7
-
 purified enzyme Cupriavidus necator

Storage Stability

EC Number Storage Stability Organism
1.2.5.2 -80°C, stable for prolonged periods in 20 mM Tris-HCl, pH 8.0, containing 5 mM CaCl2 Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.5.2 acetaldehyde + 2 ferricyanide + H2O
-
 Cupriavidus necator acetate + 2 ferrocyanide + 2 H+
-
 ?
1.2.5.2 butanal + 2 ferricyanide + H2O
-
 Cupriavidus necator butanoate + 2 ferrocyanide + 2 H+
-
 ?
1.2.5.2 formaldehyde + 2 ferricyanide + H2O
-
 Cupriavidus necator formate + 2 ferrocyanide + 2 H+
-
 ?
1.2.5.2 furfural + 2 ferricyanide + H2O
-
 Cupriavidus necator 2-furoic acid + 2 ferrocyanide + 2 H+
-
 ?
1.2.5.2 additional information product determination, overview. The enzyme shows broad substrate specificity converting different primary alcohols, starting from C2 compounds, secondary alcohols, diols, polyethylene glycol 6000, and aldehydes, including formaldehyde, detailed overview Cupriavidus necator ?
-
 ?
1.2.5.2 pentanal + 2 ferricyanide + H2O
-
 Cupriavidus necator pentanoate + 2 ferrocyanide + 2 H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.5.2 monomer 1 * 73600, SDS-PAGE Cupriavidus necator

Synonyms

EC Number Synonyms Comment Organism
1.1.5.5 pyrroloquinoline quinone-dependent alcohol dehydrogenase inducible
-
1.1.5.5 THFA-DH
-
-

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.5.2 heme c the quinohemoprotein contains one covalently bound heme c per monomer Cupriavidus necator
1.2.5.2 pyrroloquinoline quinone the quinohemoprotein contains one pyrroloquinoline quinone per monomer Cupriavidus necator

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.2.5.2 Cupriavidus necator isoelectric focusing
-
 9.1