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Literature summary extracted from

  • Pernot, P.; Mothet, J.P.; Schuvailo, O.; Soldatkin, A.; Pollegioni, L.; Pilone, M.; Adeline, M.T.; Cespuglio, R.; Marinesco, S.
    Characterization of a yeast D-amino acid oxidase microbiosensor for D-serine detection in the central nervous system (2008), Anal. Chem., 80, 1589-1597.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.3 expressed in Escherichia coli Rhodotorula toruloides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.3 3.62
-
 D-Ser apparent value, immobilized enzyme Rhodotorula toruloides
1.4.3.3 13.75
-
 D-Ser apparent value, free enzyme Rhodotorula toruloides

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.3 Rhodotorula toruloides
-
-
-
1.4.3.3 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.3
-
 Rhodotorula toruloides

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.3 kidney
-
 Sus scrofa
-

Storage Stability

EC Number Storage Stability Organism
1.4.3.3 -20°C, immobilized on microbiosensor, more than 160 days, 2% loss of activity Sus scrofa
1.4.3.3 -20°C, immobilized on microbiosensor, more than 160 days, 2% loss of activity Rhodotorula toruloides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.3 D-Ala + H2O + O2 104% activity compared to D-Ser Rhodotorula toruloides pyruvate + NH3 + H2O2
-
 ?
1.4.3.3 D-Ala + H2O + O2 165% activity compared to D-Ser Sus scrofa pyruvate + NH3 + H2O2
-
 ?
1.4.3.3 D-Asp + H2O + O2 38.6% activity compared to D-Ser Sus scrofa oxaloacetate + NH3 + H2O2
-
 ?
1.4.3.3 D-Asp + H2O + O2 5.5% activity compared to D-Ser Rhodotorula toruloides oxaloacetate + NH3 + H2O2
-
 ?
1.4.3.3 D-Ser + H2O + O2 100% activity Sus scrofa 2-oxo-3-hydroxypropionate + NH3 + H2O2
-
 ?
1.4.3.3 D-Ser + H2O + O2 100% activity Rhodotorula toruloides 2-oxo-3-hydroxypropionate + NH3 + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.3.3 D-amino acid oxidase
-
 Sus scrofa
1.4.3.3 D-amino acid oxidase
-
 Rhodotorula toruloides
1.4.3.3 DAAO
-
 Sus scrofa
1.4.3.3 DAAO
-
 Rhodotorula toruloides

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.3 15 40 the response to D-serine increases with temperature, from 15 to 40°C, the sensitivity at 37°C is 75% higher than that at 25°C Rhodotorula toruloides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.3 8 8.5 immobilized enzyme Rhodotorula toruloides

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.3.3 5.4 8.5 the response to 0.01 mM D-serine increases from pH 5.4 to 8.0, reaches a maximum at pH 8.0-8.5, and decreases at higher values, the response at physiological pH (7.4) is 80.7% of the maximum response observed at pH 8.5 Rhodotorula toruloides

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.3 FAD
-
 Sus scrofa
1.4.3.3 FAD
-
 Rhodotorula toruloides