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Literature summary extracted from

  • Kurtovic, S.; Grehn, L.; Karlsson, A.; Hellman, U.; Mannervik, B.
    Glutathione transferase activity with a novel substrate mimics the activation of the prodrug azathioprine (2008), Anal. Biochem., 375, 339-344.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.18 expressed in Escherichia coli Homo sapiens
2.5.1.18 expressed in Escherichia coli Bos taurus
2.5.1.18 expressed in Escherichia coli Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Bos taurus
-
-
-
2.5.1.18 Homo sapiens
-
-
-
2.5.1.18 Homo sapiens P08263
-
-
2.5.1.18 Homo sapiens Q16772
-
-
2.5.1.18 Rattus norvegicus P04903
-
-
2.5.1.18 Rattus norvegicus P04904
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18 nickel-immobilized metal ion affinity chromatography Homo sapiens
2.5.1.18 nickel-immobilized metal ion affinity chromatography Bos taurus
2.5.1.18 nickel-immobilized metal ion affinity chromatography Rattus norvegicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.18 0.005
-
 isozyme GST A1-1, using azathioprine as a substrate Bos taurus
2.5.1.18 0.005
-
 isozyme GST A2-2, using azathioprine as a substrate Rattus norvegicus
2.5.1.18 0.01
-
 isozyme GST A3-3, using azathioprine as a substrate Homo sapiens
2.5.1.18 0.023
-
 isozyme GST A3-3, using azathioprine as a substrate Rattus norvegicus
2.5.1.18 0.035
-
 isozyme GST A3-3, using 1-methyl-4-nitro-5-(4-nitrophenylthio)-1H-imidazole as a substrate Rattus norvegicus
2.5.1.18 0.21
-
 isozyme GST A1-1, using azathioprine as a substrate Homo sapiens
2.5.1.18 0.53
-
 isozyme GST A2-2, using azathioprine as a substrate Homo sapiens
2.5.1.18 2.57
-
 isozyme GST A2-2, using 1-methyl-4-nitro-5-(4-nitrophenylthio)-1H-imidazole as a substrate Rattus norvegicus
2.5.1.18 4.58
-
 isozyme GST A1-1, using 1-methyl-4-nitro-5-(4-nitrophenylthio)-1H-imidazole as a substrate Bos taurus
2.5.1.18 7.26
-
 isozyme GST A3-3, using 1-methyl-4-nitro-5-(4-nitrophenylthio)-1H-imidazole as a substrate Homo sapiens
2.5.1.18 53.2
-
 isozyme GST A2-2, using 1-methyl-4-nitro-5-(4-nitrophenylthio)-1H-imidazole as a substrate Homo sapiens
2.5.1.18 61.8
-
 isozyme GST A1-1, using 1-methyl-4-nitro-5-(4-nitrophenylthio)-1H-imidazole as a substrate Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 glutathione + 1-methyl-4-nitro-5-(4-nitrophenylthio)-1H-imidazole
-
 Homo sapiens ?
-
 ?
2.5.1.18 glutathione + 1-methyl-4-nitro-5-(4-nitrophenylthio)-1H-imidazole
-
 Bos taurus ?
-
 ?
2.5.1.18 glutathione + 1-methyl-4-nitro-5-(4-nitrophenylthio)-1H-imidazole
-
 Rattus norvegicus ?
-
 ?
2.5.1.18 glutathione + azathioprine
-
 Homo sapiens 6-mercaptopurine + ?
-
 ?
2.5.1.18 glutathione + azathioprine
-
 Bos taurus 6-mercaptopurine + ?
-
 ?
2.5.1.18 glutathione + azathioprine
-
 Rattus norvegicus 6-mercaptopurine + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 glutathione S-transferase
-
 Homo sapiens
2.5.1.18 glutathione S-transferase
-
 Bos taurus
2.5.1.18 glutathione S-transferase
-
 Rattus norvegicus
2.5.1.18 GST A1-1 isozyme Bos taurus
2.5.1.18 GST A1-1 isozyme Homo sapiens
2.5.1.18 GST A2-2 isozyme Homo sapiens
2.5.1.18 GST A2-2 isozyme Rattus norvegicus
2.5.1.18 GST A3-3 isozyme Homo sapiens
2.5.1.18 GST A3-3 isozyme Rattus norvegicus