Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zendman, A.J.; Raijmakers, R.; Nijenhuis, S.; Vossenaar, E.R.; Tillaart, M.; Chirivi, R.G.; Raats, J.M.; van Venrooij, W.J.; Drijfhout, J.W.; Pruijn, G.J.
    ABAP: antibody-based assay for peptidylarginine deiminase activity (2007), Anal. Biochem., 369, 232-240.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.3.15 PAD2 and PAD4 from spleen, expression of N-terminally His-tagged enzymes in Escherichia coli strain BL21(DE3) Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.3.15 EDTA
-
 Homo sapiens
3.5.3.15 EDTA
-
 Mus musculus
3.5.3.15 EDTA
-
 Oryctolagus cuniculus
3.5.3.15 N-alpha-benzoyl-N5-(2-chloro-1-iminoethyl)-L-ornithine amide
-
 Homo sapiens
3.5.3.15 N-alpha-benzoyl-N5-(2-chloro-1-iminoethyl)-L-ornithine amide
-
 Mus musculus
3.5.3.15 N-alpha-benzoyl-N5-(2-chloro-1-iminoethyl)-L-ornithine amide
-
 Oryctolagus cuniculus
3.5.3.15 N-alpha-benzoyl-N5-(2-chloro-1-iminoethyl)-L-ornithine ethyl ester complete inhibition of recombinant PAD4 at 2 mM Homo sapiens
3.5.3.15 N-alpha-benzoyl-N5-(2-chloro-1-iminoethyl)-L-ornithine ethyl ester
-
 Mus musculus
3.5.3.15 N-alpha-benzoyl-N5-(2-chloro-1-iminoethyl)-L-ornithine ethyl ester
-
 Oryctolagus cuniculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.3.15 Ca2+ required Mus musculus
3.5.3.15 Ca2+ required Oryctolagus cuniculus
3.5.3.15 Ca2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.3.15 protein-L-arginine + H2O Mus musculus
-
 protein-L-citrulline + NH3
-
 ?
3.5.3.15 protein-L-arginine + H2O Oryctolagus cuniculus
-
 protein-L-citrulline + NH3
-
 ?
3.5.3.15 protein-L-arginine + H2O Homo sapiens
-
 protein-L-citrulline + NH3
-
 ?
3.5.3.15 protein-L-arginine + H2O Mus musculus C57BL/6 / 129S
-
 protein-L-citrulline + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.15 Homo sapiens Q9Y2J8 PAD2; isozyme PAD2
-
3.5.3.15 Mus musculus
-
 C57BL/6-129S mice
-
3.5.3.15 Mus musculus C57BL/6 / 129S
-
 C57BL/6-129S mice
-
3.5.3.15 Oryctolagus cuniculus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.3.15 brain low activity Mus musculus
-
3.5.3.15 kidney low activity Mus musculus
-
3.5.3.15 lung low activity Mus musculus
-
3.5.3.15 additional information tissue distribution, no activity in testis and liver, overview Mus musculus
-
3.5.3.15 muscle high activity Mus musculus
-
3.5.3.15 ovary
-
 Mus musculus
-
3.5.3.15 skeletal muscle
-
 Oryctolagus cuniculus
-
3.5.3.15 spinal cord
-
 Mus musculus
-
3.5.3.15 spleen low activity Mus musculus
-
3.5.3.15 spleen PAD2 and PAD4 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.3.15 additional information
-
 development and evaluation of a highly sensitive antibody-based assay method for PAD activity determination, calibration using PAD from rabbit skeletal muscle, overview Oryctolagus cuniculus
3.5.3.15 additional information
-
 development and evaluation of a highly sensitive antibody-based assay method for PAD activity determination, overview Mus musculus
3.5.3.15 additional information
-
 development and evaluation of a highly sensitive antibody-based assay method for PAD activity determination, overview Homo sapiens
3.5.3.15 10.14
-
 muscle enzyme Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.15 N-alpha-benzoyl-L-arginine ethyl ester + H2O
-
 Mus musculus N-alpha-benzoyl-L-citrulline ethyl ester + NH3
-
 ?
3.5.3.15 N-alpha-benzoyl-L-arginine ethyl ester + H2O
-
 Oryctolagus cuniculus N-alpha-benzoyl-L-citrulline ethyl ester + NH3
-
 ?
3.5.3.15 N-alpha-benzoyl-L-arginine ethyl ester + H2O
-
 Homo sapiens N-alpha-benzoyl-L-citrulline ethyl ester + NH3
-
 ?
3.5.3.15 N-alpha-benzoyl-L-arginine ethyl ester + H2O
-
 Mus musculus C57BL/6 / 129S N-alpha-benzoyl-L-citrulline ethyl ester + NH3
-
 ?
3.5.3.15 protein-L-arginine + H2O
-
 Mus musculus protein-L-citrulline + NH3
-
 ?
3.5.3.15 protein-L-arginine + H2O
-
 Oryctolagus cuniculus protein-L-citrulline + NH3
-
 ?
3.5.3.15 protein-L-arginine + H2O
-
 Homo sapiens protein-L-citrulline + NH3
-
 ?
3.5.3.15 protein-L-arginine + H2O development and evaluation of a highly sensitive antibody-based assay method for PAD activity determination, overview Mus musculus protein-L-citrulline + NH3
-
 ?
3.5.3.15 protein-L-arginine + H2O development and evaluation of a highly sensitive antibody-based assay method for PAD activity determination, overview Oryctolagus cuniculus protein-L-citrulline + NH3
-
 ?
3.5.3.15 protein-L-arginine + H2O development and evaluation of a highly sensitive antibody-based assay method for PAD activity determination, overview Homo sapiens protein-L-citrulline + NH3
-
 ?
3.5.3.15 protein-L-arginine + H2O
-
 Mus musculus C57BL/6 / 129S protein-L-citrulline + NH3
-
 ?
3.5.3.15 protein-L-arginine + H2O development and evaluation of a highly sensitive antibody-based assay method for PAD activity determination, overview Mus musculus C57BL/6 / 129S protein-L-citrulline + NH3
-
 ?
3.5.3.15 SHQESTRGKSKGKAAAAA + H2O
-
 Homo sapiens SHQEST-Cit-GKSKGKAAAAA + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.3.15 PAD
-
 Mus musculus
3.5.3.15 PAD
-
 Oryctolagus cuniculus
3.5.3.15 PAD
-
 Homo sapiens
3.5.3.15 peptidylarginine deiminase
-
 Mus musculus
3.5.3.15 peptidylarginine deiminase
-
 Oryctolagus cuniculus
3.5.3.15 peptidylarginine deiminase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.3.15 37
-
 assay at Mus musculus
3.5.3.15 37
-
 assay at Oryctolagus cuniculus
3.5.3.15 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.15 7.6
-
 assay at Mus musculus
3.5.3.15 7.6
-
 assay at Oryctolagus cuniculus
3.5.3.15 7.6
-
 assay at Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.5.3.15 0.5
-
 recombinant PAD4, pH 7.6, 37°C Homo sapiens N-alpha-benzoyl-N5-(2-chloro-1-iminoethyl)-L-ornithine ethyl ester