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Literature summary extracted from

  • Mahmoud, A.E.; Abdou, H.M.; Jwanny, E.W.
    Extracellular Pleurotus ostreatus cholesterol oxidase. Purification and characterization (2007), Adv. Food Sci., 29, 12-20.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.6 CuSO4 1 mM, complete inhibition Pleurotus ostreatus
1.1.3.6 MgCl2 1 mM, complete inhibition Pleurotus ostreatus
1.1.3.6 p-chloromercuribenzoate 0.01 mM, complete inhibition Pleurotus ostreatus
1.1.3.6 Pb(OAc)2 1 mM, complete inhibition Pleurotus ostreatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.6 0.25
-
 cholesterol
-
 Pleurotus ostreatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.3.6 extracellular
-
 Pleurotus ostreatus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.6 50000
-
 x * 50000, SDS-PAGE Pleurotus ostreatus

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.6 Pleurotus ostreatus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.6
-
 Pleurotus ostreatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.6 culture condition the strain reaches its maximal utilization of cholesterol as the only C source for production of extracellular cholesterol oxidase Pleurotus ostreatus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.6 cholesterol + O2
-
 Pleurotus ostreatus cholest-4-en-3-one + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.6 ? x * 50000, SDS-PAGE Pleurotus ostreatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.6 60
-
-
 Pleurotus ostreatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.6 50
-
 60 min, stable Pleurotus ostreatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.6 7
-
-
 Pleurotus ostreatus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.6 5 7 stable at Pleurotus ostreatus