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Literature summary extracted from
- Structure of the T109S mutant of Escherichia coli dihydroorotase complexed with the inhibitor 5-fluoroorotate: catalytic activity is reflected by the crystal form (2007), Acta Crystallogr. Sect. F, F63, 154-161.
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.5.2.3 | in complex with 5-fluoroorotate, hanging drop vapour diffusion method 14-16% PEG 3350, 0.1 M MES pH 6.25, 25 mM MgCl2, 0.2 M KCl and 30% sucrose | Escherichia coli |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.5.2.3 | T109S | diminished activity | Escherichia coli |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.2.3 | 5-Fluoroorotate | - |
Escherichia coli |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.2.3 | Escherichia coli | P05020 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.2.3 | L-dihydroorotate + H2O | - |
Escherichia coli | N-carbamoyl-L-aspartate | - |
r | |
| 3.5.2.3 | N-carbamoyl-L-aspartate | - |
Escherichia coli | L-dihydroorotate + H2O | - |
r | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.2.3 | DHOase | - |
Escherichia coli |
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Release 2023.1 (January 2023)
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