Literature summary extracted from

Puttick, J.; Vieille, C.; Song, S.H.; Fodje, M.N.; Grochulski, P.; Delbaere, L.T.
Crystallization, preliminary X-ray diffraction and structure analysis of Thermotoga maritima mannitol dehydrogenase (2007), Acta Crystallogr. Sect. F, 63, 350-352.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.1.1.67	gene mtdh, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3)	Thermotoga maritima

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
1.1.1.67	purified recombinant His-tagged enzyme, 10 mg/ml protein in 50 mM Tris-HCl, pH 8.5, room temperature, microbatch-under-oil method, mixing of equal volumes of protein and crystallization solutions, the latter containing 30% 2-methyl-2,4-pentanediol, and 0.1 M Na HEPES, pH 7.5, X-ray diffraction structure determination and analysis at 3.3 A resolution, molecular-replacement	Thermotoga maritima

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.1.1.67	Thermotoga maritima	-	gene mtdh	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.1.1.67	recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography	Thermotoga maritima

Synonyms

EC Number	Synonyms	Comment	Organism
1.1.1.67	mannitol dehydrogenase	-	Thermotoga maritima
1.1.1.67	MtDH	-	Thermotoga maritima

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Release 2023.1 (January 2023)