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Literature summary extracted from

  • Viola, R.E.; Liu, X.; Ohren, J.F.; Faehnle, C.R.
    The structure of a redundant enzyme: a second isoform of aspartate beta-semialdehyde dehydrogenase in Vibrio cholerae (2008), Acta Crystallogr. Sect. D, D64, 321-330.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.2.1.11 drug development ASADH is a target for the development of novel antibiotics, especially for Gram-negative bacteria that require diaminopimelate for cell-wall biosynthesis Vibrio cholerae serotype O1

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.11 expression in Escherichia coli Vibrio cholerae serotype O1

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.2.1.11 apoenzyme and enzyme in complex with substrate L-aspartate semialdehyde, method optimization, purified protein in 50 mM sodium citrate, pH 5.6, with 0.2 M ammonium acetate and 2 mM DTT via dialysis overnight, hanging drop vapour diffusion method, 4°C, diluted back into 100 mM Tris, pH 8.5, with 200 mM ammonium acetate and 5 mM DTT with 12 mg/ml protein, 0.001 ml protein solution is mixed with 0.001 ml of precipitant containing 0.1 M sodium citrate, pH 5.5-6.5, 5 mM DTT, 0.1-0.4 M ammonium acetate, and 24-27% PEG 8000, method optimization, overview, X-ray diffraction structure determination and analysis at 2.0-2.2 A resolution, molecular replacement method Vibrio cholerae serotype O1

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.11 L-aspartate 4-semialdehyde + phosphate + NADP+ Vibrio cholerae serotype O1 the essential ASADH produces the first branch-point metabolite in the biosynthetic pathways that lead to the production of lysine, threonine, methionine and isoleucine as well as the cell-wall precursor diaminopimelate L-4-aspartyl phosphate + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.11 Vibrio cholerae serotype O1
-
 isozymes ASADH1 and ASADH2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.11 recombinant enzyme from Escherichia coli to over 95% purity by anion exchange chromatography and gel filtration Vibrio cholerae serotype O1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.11 L-aspartate 4-semialdehyde + phosphate + NADP+
-
 Vibrio cholerae serotype O1 L-4-aspartyl phosphate + NADPH + H+
-
 ?
1.2.1.11 L-aspartate 4-semialdehyde + phosphate + NADP+ the essential ASADH produces the first branch-point metabolite in the biosynthetic pathways that lead to the production of lysine, threonine, methionine and isoleucine as well as the cell-wall precursor diaminopimelate Vibrio cholerae serotype O1 L-4-aspartyl phosphate + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.11 dimer
-
 Vibrio cholerae serotype O1

Synonyms

EC Number Synonyms Comment Organism
1.2.1.11 ASADH
-
 Vibrio cholerae serotype O1
1.2.1.11 aspartate beta-semialdehyde dehydrogenase
-
 Vibrio cholerae serotype O1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.11 8.5
-
-
 Vibrio cholerae serotype O1

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.11 NADP+
-
 Vibrio cholerae serotype O1